Novel multiple-locus variable-number tandem-repeat analysis method for rapid molecular typing of human Staphylococcus aureus

被引：20
作者：
Ikawaty, Risma [1 ]
Willems, Rob J. L. [1 ]
Box, Adrienne T. A. [1 ]
Verhoef, Jan [1 ]
Fluit, Ad C. [1 ]
机构：
[1] Univ Med Ctr Utrecht, Dept Med Microbiol, NL-3584 CX Utrecht, Netherlands
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 09期
关键词：
D O I：
10.1128/JCM.00861-08
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We evaluated the use of a novel multiple-locus variable-number tandem-repeat analysis (MLVA) method for typing of human Staphylococcus aureus. For a total of 150 clinical isolates, MLVA demonstrated the highest discriminatory power. MLVA correctly assigned isolates to outbreaks or identified isolates as unlinked. MLVA is a rapid and simple method for the epidemiological typing of S. aureus.
引用
收藏
页码：3147 / 3151
页数：5
相关论文
共 13 条
[1]   PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS [J].
BANNERMAN, TL ;
HANCOCK, GA ;
TENOVER, FC ;
MILLER, JM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :551-555
[2]   Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus [J].
Enright, MC ;
Day, NPJ ;
Davies, CE ;
Peacock, SJ ;
Spratt, BG .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) :1008-1015
[3]   Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates [J].
Francois, P ;
Huyghe, A ;
Charbonnier, Y ;
Bento, M ;
Herzig, S ;
Topolski, I ;
Fleury, N ;
Lew, D ;
Vaudaux, P ;
Harbarth, S ;
van Leeuwen, W ;
van Belkum, A ;
Blanc, DS ;
Pittet, D ;
Schrenzel, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (07) :3346-3355
[4]   Differentiation of bovine Staphylococcus aureus isolates by use,of polymorphic tandem repeat typing [J].
Gilbert, Florence B. ;
Fromageau, Angelina ;
Gelineau, Ludovic ;
Poutrel, Bernard .
VETERINARY MICROBIOLOGY, 2006, 117 (2-4) :297-303
[5]   Use of variations in Staphylococcal interspersed repeat units for molecular typing of methicillin-resistant Staphylococcus aureus strains [J].
Hardy, KJ ;
Oppenheim, BA ;
Gossain, S ;
Gao, F ;
Hawkey, PM .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (01) :271-273
[6]   Distribution and characterization of staphylococcal interspersed repeat units (SIRUs) and potential use for strain differentiation [J].
Hardy, KJ ;
Ussery, DW ;
Oppenheim, BA ;
Hawkey, PM .
MICROBIOLOGY-SGM, 2004, 150 :4045-4052
[7]   Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management [J].
Harmsen, D ;
Claus, H ;
Witte, W ;
Rothgänger, J ;
Claus, H ;
Turnwald, D ;
Vogel, U .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) :5442-5448
[8]   Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria [J].
Lindstedt, BA .
ELECTROPHORESIS, 2005, 26 (13) :2567-2582
[9]   Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing for clonal characterization of Staphylococcus aureus isolates [J].
Malachowa, N ;
Sabat, A ;
Gniadkowski, M ;
Krzyszton-Russjan, J ;
Empel, J ;
Miedzobrodzki, J ;
Kosowska-Shick, K ;
Appelbaum, PC ;
Hryniewicz, W .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (07) :3095-3100
[10]   New method for typing Staphylococcus aureus strains:: Multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates [J].
Sabat, A ;
Krzyszton-Russjan, J ;
Strzalka, W ;
Filipek, R ;
Kosowska, K ;
Hryniewicz, W ;
Travis, J ;
Potempa, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (04) :1801-1804
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