Expression of Proliferation Cell Nuclear Antigen (PCNA) in Peripheral Erythrocytes of Triploid Rainbow Trout Oncorhynchus mykiss (Walbaum)

被引：0

作者：

Liu, Yi ^{[1
]}

Wang, Bingqian ^{[2
]}

Fan, Zhaoting ^{[1
]}

Liu, Haijin ^{[1
]}

机构：

[1] Northeast Agr Univ, Sch Anim Sci & Technol, Harbin 150030, Peoples R China

[2] Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin 150070, Peoples R China

来源：

JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2012年 / 11卷 / 23期

关键词：

Rainbow trout; triploid; PCNA; immature erythrocyte; erythroid cells; China; ATLANTIC SALMON; DIVISION; GROWTH; BLOOD;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The proliferation of erythroid cells in. peripheral blood was detected by anti-PCNA monoclonal antibody (PC10) with a means of immunocytochemistry in. diploid and triploid rainbow trout. The rate of PCNA positive erythroid cells in triploidy peripheral blood was detected by flow cytometry. The erythrocytes which were positive for PCNA were confined in the cells with high nuclear-cytoplasmic ratio. About 6.4% erythrocytes were found positive for PCNA in triploid rainbow trout while only 0.4% in. diploids. And the PCNA positive rate of the erythrocytes was 0.37% in. diploids and 8.39% in triploids in average. PCNA, a proliferating marker is found to be expressed only in immature cells in the peripheral erythrocytes of triploid rainbow trout This suggests that peripheral erythrocytes with the ability of proliferating are immature cells.

引用

页码：4451 / 4454

页数：4

共 15 条

[1]

Babin Patrick J., 2007, P39, DOI 10.1007/978-1-4020-6235-3_2

[2] MOLECULAR-CLONING, STRUCTURE AND EXPRESSION OF THE YEAST PROLIFERATING CELL NUCLEAR ANTIGEN GENE [J].

BAUER, GA ;

BURGERS, PMJ .

NUCLEIC ACIDS RESEARCH, 1990, 18 (02) :261-265

[3]

Benfey Tillmann J., 1999, Reviews in Fisheries Science, V7, P39, DOI 10.1080/10641269991319162

[4] BINUCLEATED RED-BLOOD-CELLS IN THE PERIPHERAL-BLOOD OF AN ATLANTIC SALMON, SALMO-SALAR L, ALEVIN [J].

BENFEY, TJ ;

SUTTERLIN, AM .

JOURNAL OF FISH DISEASES, 1984, 7 (05) :415-420

[5] Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss) [J].

Bouraoui, L. ;

Gutierrez, J. ;

Navarro, I. .

JOURNAL OF ENDOCRINOLOGY, 2008, 198 (03) :459-469

[6] CHANGES IN THE POPULATION OF POLYRIBOSOMAL CONTAINING RED-CELLS OF PERIPHERAL-BLOOD OF RAINBOW-TROUT, SALMO-GAIRDNERI RICHARDSON, FOLLOWING STARVATION AND BLEEDING [J].

LANE, HC ;

THARP, TP .

JOURNAL OF FISH BIOLOGY, 1980, 17 (01) :75-81

[7] Proliferating cell nuclear antigen (PCNA) as a proliferative marker during embryonic and adult zebrafish hematopoiesis [J].

Leung, AYH ;

Leung, JCK ;

Chan, LYY ;

Ma, ESK ;

Kwan, TTF ;

Lai, KN ;

Meng, A ;

Liang, R .

HISTOCHEMISTRY AND CELL BIOLOGY, 2005, 124 (02) :105-111

[8] GROWTH-CHARACTERISTICS OF PHYTOPLANKTON DETERMINED BY CELL-CYCLE PROTEINS - PCNA IMMUNOSTAINING OF DUNALIELLA-TERTIOLECTA (CHLOROPHYCEAE) [J].

LIN, SJ ;

CHANG, J ;

CARPENTER, EJ .

JOURNAL OF PHYCOLOGY, 1995, 31 (03) :388-395

[9] Transcriptome profiling the gills of amoebic gill disease (AGD)-affected Atlantic salmon (Salmo salar L.):: a role for tumor suppressor p53 in AGD pathogenesis? [J].

Morrison, Richard N. ;

Cooper, Glenn A. ;

Koop, Ben F. ;

Rise, Matthew L. ;

Bridle, Andrew R. ;

Adams, Mark B. ;

Nowak, Barbara F. .

PHYSIOLOGICAL GENOMICS, 2006, 26 (01) :15-34

[10] DIVISION OF GOLDFISH ERYTHROCYTES IN CIRCULATION [J].

MURAD, A ;

EVERILL, S ;

HOUSTON, A .

CANADIAN JOURNAL OF ZOOLOGY, 1993, 71 (11) :2190-2198

← 1 2 →