Extracellular polysaccharide from Weissella confusa OF126: Production, optimization, and characterization

被引:55
作者
Adesulu-Dahunsi, A. T. [1 ,2 ]
Sanni, A. I. [1 ]
Jeyaram, K. [2 ]
Ojediran, J. O. [3 ]
Ogunsakin, A. O. [1 ,4 ]
Banwo, K. [1 ]
机构
[1] Univ Ibadan, Dept Microbiol, Ibadan, Oyo State, Nigeria
[2] IBSD, Microbial Resources Div, Imphal 795001, Manipur, India
[3] Landmark Univ, Dept Agr & Biosyst Engn, Omu Aran, Kwara State, Nigeria
[4] CSIR, Cent Food Technol Res Inst, FMBCT Dept, Mysore 570020, Karnataka, India
关键词
Exopolysaccharide; Probiotic; FTIR; LACTIC-ACID BACTERIA; LACTOBACILLUS-PLANTARUM STRAINS; INDIGENOUS FERMENTED FOODS; IN-VITRO; EXOPOLYSACCHARIDE PRODUCTION; ANTIOXIDANT ACTIVITIES; BIOACTIVITIES; IDENTIFICATION; PROBIOTICS; SELECTION;
D O I
10.1016/j.ijbiomac.2018.01.060
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The production, optimization, and characterization of exopolysaccharide (EPS) from Weissella confusa OF126 and the in-vitro probiotic potentials of this strain was investigated. The EPS produced on sucrose modified-MRS broth was characterized. The purified EPS had an average molecular weight of 1.1 x 10(6) Da. HPLC analysis revealed the presence of glucose monomers, indicating its homopolysaccharide nature. The structural characteristics of the EPS were investigated by FTIR, and NMR spectroscopy. FTIR spectroscopy revealed the presence of hydroxyl, carboxyl, N-acetyl and amine groups. NMR analysis confirmed that the EPS contained alpha-(1 -> 6) linkage and alpha-(1 -> 3) branched linkage. The EPS showed strong in-vitro antioxidant activity. Four significant factors were optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM). The predicted optimum conditions for EPS production were cultivation time (48.50 h), sucrose concentration (24.00 g/L), pH (7.00) and yeast extract (2.50%).The EPS produced was predicted to be 3.10 g/L, while the experimental yield was 3.00 g/L. This strain was found to possess desirable probiotic attributes by its ability to survive at pH 2.0 and in the presence of bile salts (0.50% (w/v)) for 4 h. The results obtained from this study demonstrate W. confusa OF126 as a promising probiotic and the EPS produced can find useful applications in industries. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码:514 / 525
页数:12
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