Preliminary molecular analysis of Clostridium difficile isolates from healthy horses in northern Italy

被引：13

作者：

Ossiprandi, Maria Cristina ^{[1
]}

Buttrini, Mirko ^{[2
]}

Bottarelli, Ezio ^{[1
]}

Zerbini, Laura ^{[1
]}

机构：

[1] Univ Parma, Fac Vet Med, Dept Anim Hlth, I-43100 Parma, Italy

[2] Univ Parma, Fac Med & Surg, Dept Pathol & Lab Med, I-43100 Parma, Italy

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2010年 / 33卷 / 06期

关键词：

Clostridium difficile; Molecular analysis; Healthy horses; Zoonosis; Toxigenic/non-toxigenic;

D O I：

10.1016/j.cimid.2009.10.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Clostridium difficile, associated with a wide spectrum of diseases in humans, as well as in several animal species, is an important cause of colitis in adult horses and foals. The aim of this study was to investigate by toxin gene profile and PCR-ribotyping the molecular characteristics of 14 C. difficile strains isolated from 42 faeces of healthy horses. Both toxin genes, tcdA and tcdB, were present in only 1 isolate (7.1%). Six isolates (42.9%) demonstrated tcdA-/tcdB+ genotype, and seven isolates (50.0%) were tcdA-/tcdB-. All strains were binary toxin genes negative (cdtA-/cdtB-). The PCR-positive strains, except for the tcdA+/tcdB+ isolate, tested negative for, in vitro, A and/or B toxins production by EIA. Eleven distinct ribotypes were observed. In conclusion, C. difficile can be present in the normal intestinal flora of healthy adult horses, in addition to foals. These animals could therefore play an important role as potential reservoirs of toxigenic strains. Published by Elsevier B.V.

引用

页码：E25 / E29

页数：5

共 28 条

[1] PCR ribotyping of Clostridium difficile isolates originating from human and animal sources
Arroyo, LG
Kruth, SA
Willey, BM
Staempfli, HR
Low, DE
Weese, JS
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (02) : 163 - 166
[2] Molecular analysis of Clostridium difficile isolates recovered from horses with diarrhea
Arroyo, Luis G.
Staempfli, Henry
Weese, J. Scott
[J]. VETERINARY MICROBIOLOGY, 2007, 120 (1-2) : 179 - 183
[3] Prevalence and genetic characterization of toxin A variant strains of Clostridium difficile among adults and children with diarrhea in France
Barbut, F
Lalande, V
Burghoffer, B
Thien, HV
Grimprel, E
Petit, JC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) : 2079 - 2083
[4] Epidemiology of Clostridium difficile-associated infections
Barbut, F
Petit, JC
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2001, 7 (08) : 405 - 410
[5] Clinical features of Clostridium difficile-associated infections and molecular characterization of strains:: Results of a retrospective study, 2000-2004
Barbut, Frederic
Gariazzo, Beatrice
Bonne, Laetitia
Lalande, Valerie
Burghoffer, Beatrice
Luiuz, Ralucca
Petit, Jean-Claude
[J]. INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2007, 28 (02) : 131 - 139
[6] Clostridum difficile diarrhea:: infection control in horses
Båverud, V
[J]. VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, 2004, 20 (03) : 615 - +
[7] Clostridium difficile:: prevalence in horses and environment, and antimicrobial susceptibility
Bâverud, V
Gustafsson, A
Franklin, A
Aspán, A
Gunnarsson, A
[J]. EQUINE VETERINARY JOURNAL, 2003, 35 (05) : 465 - 471
[8] Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile
Bidet, P
Lalande, V
Salauze, B
Burghoffer, B
Avesani, V
Delmée, M
Rossier, A
Barbut, F
Petit, JC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) : 2484 - 2487
[9] Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing
Bidet, P
Barbut, F
Lalande, V
Burghoffer, B
Petit, JC
[J]. FEMS MICROBIOLOGY LETTERS, 1999, 175 (02) : 261 - 266
[10] Laboratory diagnosis of Clostridium difficile associated diarrhoea:: a plea for culture
Delmée, M
Van Broeck, J
Simon, A
Janssens, M
Avesani, V
[J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (02) : 187 - 191

← 1 2 3 →