Rapid Decolorization of Phenolic Azo Dyes by Immobilized Laccase with Fe3O4/SiO2 Nanoparticles as Support

被引：64

作者：

Wang, Hongxia ^{[1
]}

Zhang, Wei ^{[2
]}

Zhao, Jingxiang ^{[1
]}

Xu, Lulu ^{[1
]}

Zhou, Chunyan ^{[1
]}

Chang, Lin ^{[1
]}

Wang, Liyan ^{[3
]}

机构：

[1] Harbin Normal Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Photon & Elect Bandgap Mat, Harbin 150025, Peoples R China

[2] Hebei Univ Technol, Sch Mat Sci & Engn, Tianjin 300130, Peoples R China

[3] Harbin Normal Univ, Sch Sci, Harbin 150086, Peoples R China

来源：

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2013年 / 52卷 / 12期

基金：

黑龙江省自然科学基金; 中国国家自然科学基金;

关键词：

SYNTHETIC DYES; CARBON; BIODEGRADATION; DETOXIFICATION; DEGRADATION; MEDIATORS; OXIDATION; REMOVAL; SURFACE;

D O I：

10.1021/ie302627c

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Fe3O4/SiO2 nanoparticles with particle size below 30 nm were used as the support for laccase immobilization through glutaraldehyde coupling. Investigation of the immobilized laccase was carried out by X-ray diffractometry (XRD), transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), vibrating sample magnetometry (VSM), UV-vis spectrophotometry, and cyclic voltammogram (CV) measurements. Two phenolic azo dyes, Procion Red MX-5B and azophloxine, were selected to investigate the enzyme activity of the immobilized laccase toward degradation of phenolic azo dyes. The immobilized laccase presents unusual performance for dye decolorization and easy separation with an external magnetic field. Finally, the possible mechanism for the unusual decolorization of phenolic azo dyes by the immobilized laccase is discussed.

引用

页码：4401 / 4407

页数：7

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