Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

被引：138

作者：

Shin, Eun Myoung ^{[1
]}

Zhou, Hong Yu ^{[1
,2
]}

Guo, Lian Yu ^{[1
]}

Kim, Jeong Ah ^{[3
]}

Lee, Seung Ho ^{[3
]}

Merfort, Irmgard ^{[4
]}

Kang, Sam Sik ^{[1
]}

Kim, Hak Sung ^{[5
]}

Kim, Sanghee ^{[1
]}

Kim, Yeong Shik ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Inst Nat Prod Res, Seoul 151742, South Korea

[2] Shenyang Pharmaceut Univ, Coll Pharm, Dept Pharmacol, Shenyang 110016, Peoples R China

[3] Yeungnam Univ, Coll Pharm, Kyongsan 712749, South Korea

[4] Univ Freiburg, Dept Pharmaceut Biol & Biotechnol, D-79104 Freiburg, Germany

[5] Wonkwong Univ, Coll Pharm, Iksan 570749, South Korea

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2008年 / 8卷 / 11期

关键词：

Glycyrrhizae Radix; Glycyrol; Anti-inflammatory activity; NF-kappa B; iNOS; COX-2;

D O I：

10.1016/j.intimp.2008.06.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 mu M dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimutated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1 beta and IL-6. LPS-induced NF-kappa B activation was prevented in RAW264.7 macrophages by inhibition of I-kappa B alpha phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-kappa B alpha phosphorylation. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1524 / 1532

页数：9

共 35 条

[1] Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-KB activation in RAW 264.7 cells [J].

Ahn, KS ;

Noh, EJ ;

Zhao, HL ;

Jung, SH ;

Kang, SS ;

Kim, YS .

LIFE SCIENCES, 2005, 76 (20) :2315-2328

[2] MECHANISM OF ANTIINFLAMMATORY ACTION OF GLYCYRRHIZIN - EFFECT ON NEUTROPHIL FUNCTIONS INCLUDING REACTIVE OXYGEN SPECIES GENERATION [J].

AKAMATSU, H ;

KOMURA, J ;

ASADA, Y ;

NIWA, Y .

PLANTA MEDICA, 1991, 57 (02) :119-121

[3]

Almawi WY, 2002, J LEUKOCYTE BIOL, V71, P9

[4] THE MODE OF ACTION OF ASPIRIN-LIKE DRUGS - EFFECT ON INDUCIBLE NITRIC-OXIDE SYNTHASE [J].

AMIN, AR ;

VYAS, P ;

ATTUR, M ;

LESZCZYNSKAPIZIAK, J ;

PATEL, IR ;

WEISSMANN, G ;

ABRAMSON, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :7926-7930

[5]

ATIK E, 2007, INT J INFECT DIS

[6] NF-kappa B: Ten years after [J].

Baeuerle, PA ;

Baltimore, D .

CELL, 1996, 87 (01) :13-20

[7] Molecular mechanisms of anti-inflammatory action of glucocorticoids [J].

Cato, ACB ;

Wade, E .

BIOESSAYS, 1996, 18 (05) :371-378

[8] COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: Cloning, structure, and expression [J].

Chandrasekharan, NV ;

Dai, H ;

Roos, KLT ;

Evanson, NK ;

Tomsik, J ;

Elton, TS ;

Simmons, DL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13926-13931

[9] Anti-oxidant constituents of the roots and stolons of licorice (Glycyrrhiza glabra) [J].

Chin, Young-Won ;

Jung, Hyun-Ah ;

Liu, Yue ;

Su, Bao-Ning ;

Castoro, John A. ;

Keller, William J. ;

Pereira, Michael A. ;

Kinghorn, A. Douglas .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (12) :4691-4697

[10]

Cline M J, 1970, Ser Haematol, V3, P3

← 1 2 3 4 →