A LC-MS/MS method for therapeutic drug monitoring of carbamazepine, lamotrigine and valproic acid in DBS

被引：34

作者：

Linder, Camilla ^{[1
]}

Andersson, Maria ^{[2
]}

Wide, Katarina ^{[3
,4
]}

Beck, Olof ^{[1
]}

Pohanka, Anton ^{[1
]}

机构：

[1] Karolinska Inst, Dept Lab Med, Clin Pharmacol, Stockholm, Sweden

[2] NIDA, Chem & Drug Metab Sect, NIH, Baltimore, MD USA

[3] Karolinska Univ Hosp, Dept Clin Sci, Technol & Intervent, Div Pediat, Stockholm, Sweden

[4] Karolinska Inst, Stockholm, Sweden

来源：

BIOANALYSIS | 2015年 / 7卷 / 16期

基金：

瑞典研究理事会;

关键词：

ANTIEPILEPTIC DRUGS; QUANTITATIVE BIOANALYSIS; ASSAY BIAS; CHILDREN; QUANTIFICATION; CHROMATOGRAPHY; HEMATOCRIT; SAMPLES; SALIVA;

D O I：

10.4155/bio.15.99

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Therapeutic drug monitoring of antiepileptic drugs in children with epilepsy assists for personalized drug therapy but require numerous patient visits for venous blood sampling. DBS is an alternative matrix applicable to home sampling which can save time and reduce stress for this patient group. Results: A fast LC-MS/MS method for quantification of carbamazepine, lamotrigine and valproic acid based on DBS sampling was developed. The method showed linearity in therapeutically relevant concentration ranges and compatible with unknown volume sampling and expected hematocrit range of the patient group. Conclusion: A LC-MS/MS method for the three most commonly used antiepileptic drugs has been fully validated and clinically applied on DBSs from patients at the neuropediatric clinic at Karolinska University Hospital.

引用

页码：2031 / 2039

页数：9

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