Combinatorial treatment of photothermal therapy using gold nanoshells with conventional photodynamic therapy to improve treatment efficacy: An in vitro study

被引：48

作者：

Kah, James Chen Yong ^{[2
]}

Wan, Rachel Cheng Yi ^{[3
]}

Wong, Ker Yi ^{[2
]}

Mhaisalkar, Subodh ^{[3
]}

Sheppard, Colin James Richard ^{[2
]}

Olivo, Malini ^{[1
,4
,5
]}

机构：

[1] Natl Canc Ctr, Div Med Sci, Singapore 169610, Singapore

[2] Natl Univ Singapore, Div Bioengn, Singapore 117574, Singapore

[3] Nanyang Technol Univ, Sch Mat Sci & Engn, Singapore 639798, Singapore

[4] Inst Biomed Sci, Singapore Bioimaging Consortium, Singapore 138667, Singapore

[5] Natl Univ Singapore, Dept Pharm, Singapore 117543, Singapore

来源：

LASERS IN SURGERY AND MEDICINE | 2008年 / 40卷 / 08期

关键词：

epidermal growth factor receptor (EGFR); gold nanoshells; hypericin; photodynamic therapy (PDT); photothermal therapy (PTT);

D O I：

10.1002/lsm.20674

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background and Objective: Both photodynamic (PDT) and photothermal (PTT) therapy have proven to be effective treatment strategies for cancer, but the approach of combining them into a single treatment modality may offer better treatment efficacy. We compare the treatment efficacy of such combined treatment with the individual treatment. Study Design/Materials and Methods: We perform the individual PDT, PTT and combined treatment under selected in vitro condition with our low light dose of 1.44 J/cm(2) and compare their cell viability using crystal fast violet assay. Results: Compared to PDT and PTT alone which can reduce cell viability to 30.9% and 44.0% respectively, the combined treatment under a single irradiation can further reduce the cell viability to 17.5%. Conclusion: A combined PDT and PTT treatment appears to be a more effective treatment strategy compared to conventional PDT or emerging PTT treatment.

引用

页码：584 / 589

页数：6

共 30 条

[1]

Ali SM, 2002, INT J ONCOL, V21, P531

[2] Hypericin and hypocrellin induced apoptosis in human mucosal carcinoma cells [J].

Ali, SM ;

Chee, SK ;

Yuen, GY ;

Olivo, M .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2001, 65 (01) :59-73

[3] Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity [J].

Connor, EE ;

Mwamuka, J ;

Gole, A ;

Murphy, CJ ;

Wyatt, MD .

SMALL, 2005, 1 (03) :325-327

[4] SENSITIVITY OF A MALIGNANT CELL LINE TO HYPERTHERMIA (42DEGREESC) AT LOW INTRACELLULAR PH [J].

DICKSON, JA ;

OSWALD, BE .

BRITISH JOURNAL OF CANCER, 1976, 34 (03) :262-271

[5]

FUJIMOTO S, 1994, ANTICANCER RES, V14, P67

[6] Near-infrared resonant nanoshells for combined optical imaging and photothermal cancer therapy [J].

Gobin, Andre M. ;

Lee, Min Ho ;

Halas, Naomi J. ;

James, William D. ;

Drezek, Rebekah A. ;

West, Jennifer L. .

NANO LETTERS, 2007, 7 (07) :1929-1934

[7] THE ROLE OF ENERGY IN HYPERTHERMIA-INDUCED MAMMALIAN-CELL INACTIVATION - A STUDY OF THE EFFECTS OF GLUCOSE STARVATION AND AN UNCOUPLER OF OXIDATIVE-PHOSPHORYLATION [J].

HAVEMAN, J ;

HAHN, GM .

JOURNAL OF CELLULAR PHYSIOLOGY, 1981, 107 (02) :237-241

[8] Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance [J].

Hirsch, LR ;

Stafford, RJ ;

Bankson, JA ;

Sershen, SR ;

Rivera, B ;

Price, RE ;

Hazle, JD ;

Halas, NJ ;

West, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (23) :13549-13554

[9]

HOFER KG, 1980, J NATL CANCER I, V65, P621

[10] Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods [J].

Huang, XH ;

El-Sayed, IH ;

Qian, W ;

El-Sayed, MA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (06) :2115-2120

← 1 2 3 →