TWO-LAYER SCAFFOLD PLATFORMS FOR DRUG SCREENING OF NASOPHARYNGEAL EPITHELIAL CELLS

被引：0
作者：
Lamb, Bowie P. [1 ,2 ]
Cheung, Ka Chi Sarah [2 ,3 ]
Lam, Yun Wah [2 ,3 ]
Pang, Stella W. [1 ,2 ]
机构：
[1] City Univ Hong Kong, Dept Elect Engn, Kowloon, Hong Kong, Peoples R China
[2] City Univ Hong Kong, Ctr Biosyst Neurosci & Nanotechnol, Kowloon, Hong Kong, Peoples R China
[3] City Univ Hong Kong, Dept Chem, Kowloon, Hong Kong, Peoples R China
来源：
2020 33RD IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2020) | 2020年
关键词：
3D Scaffold Platform; Cell Migration; Drug Screening; Biomimetic; NP460; Cells;
D O I：
10.1109/mems46641.2020.9056387
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
A two-layer scaffold platform was designed for drug screening without using fluorescence staining to examine the effectiveness of the tested compounds. Nasopharyngeal epithelial (NP460) cells were used in this work. Typically, NP460 cells could not squeeze into the 10 mu m narrow trenches but preferred to migrate on the top ridges. However, NP460 cells could migrate into 10 mu m wide trenches after being stimulated by transforming growth factor beta-1 (TGF-beta 1) as the cells became more active. This double-layer scaffold platform served as a drug screening device by tracking the number of cells migrated into narrow trenches after drug treatment to test and monitor the effectiveness of a compound in a biomimetic microenvironment.
引用
收藏
页码：412 / 415
页数：4
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