Alpinia oxyphylla-Schisandra chinensis Herb Pair Alleviates Amyloid-β Induced Cognitive Deficits via PI3K/Akt/Gsk-3β/CREB Pathway

被引：17

作者：

Qi, Yu ^{[1
]}

Jing, Huiting ^{[1
]}

Cheng, Xinhui ^{[1
]}

Yan, Tingxu ^{[2
]}

Xiao, Feng ^{[2
]}

Wu, Bo ^{[2
]}

Bi, Kaishun ^{[3
]}

Jia, Ying ^{[2
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Wenhua Rd 103, Shenyang 110016, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Funct Food & Wine, Wenhua Rd 103, Shenyang 110016, Peoples R China

[3] Shenyang Pharmaceut Univ, Sch Pharm, Wenhua Rd 103, Shenyang 110016, Peoples R China

来源：

NEUROMOLECULAR MEDICINE | 2020年 / 22卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Alzheimer's disease; Alpinia oxyphylla; Schisandra chinensis; Amyloid-beta; p-tau; N2a-SwedAPP cell; A-BETA; MOUSE MODEL; HYDROGEN-SULFIDE; ALZHEIMERS; PROTEIN; MEMORY; IMPAIRMENT; CELLS; NEURODEGENERATION; PHOSPHORYLATION;

D O I：

10.1007/s12017-020-08595-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Alzheimer's disease (AD), one of the most common neurodegenerative diseases, threatens people's health. Based on the theory of traditional Chinese medicine (TCM) efficacy and treatment theory, we first proposed theAlpinia oxyphylla-Schisandra chinensisherb pair (ASHP) for finding a candidate of AD treatment. This study aimed at exploring the effects of ASHP on improving the cognitive function and neurodegeneration, and revealing the possible mechanism. In this study, an amyloid-beta (A beta) induced AD model was established in mice via intracerebroventricular injection. The Y-maze test and Morris water maze test were carried out to observe the behavioral change of mice, which showed that ASHP significantly ameliorated cognitive impairment. In addition, ASHP reduced amyloid-beta deposition and downregulated the hyperphosphorylation of tau via immunofluorescence assay and western blot analysis, respectively. Subsequently we focused on the PI3K/Akt pathway that is a classical pathway related to nervous system diseases. It also noticeably ASHP improved the histopathological changes in the hippocampus and cortex. Moreover, it was found that ASHP could upregulate the PI3K/Akt/Gsk-3 beta/CREB signaling pathway in N2a-SwedAPP cells. Taken together, it suggests that ASHP might reverse cognitive deficits and neurodegeneration via PI3K/Akt/Gsk-3 beta/CREB pathway.

引用

页码：370 / 383

页数：14

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