Drug Modulation of Transductor Function of Na+,K+-ATPase

被引：1

作者：

Lopatina, E. V. ^{[1
]}

Pennyaynen, V. A. ^{[2
]}

Rogachevskyi, I. V. ^{[2
]}

Krylov, B. V. ^{[2
]}

机构：

[1] Fed Agcy Med Technol, VA Almazov Fed Ctr Heart Blood & Endocrinol, Dept Expt Pharmacol, St Petersburg, Russia

[2] Russian Acad Sci, IP Pavlov Physiol Inst, Lab Physiol Stimulated Membranes, St Petersburg 196140, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2008年 / 146卷 / 04期

基金：

俄罗斯基础研究基金会;

关键词：

organotypical tissue culture; ouabain; EGTA; Na+; K+-ATPase; signal transductor; quantum-chemical estimations;

D O I：

10.1007/s10517-009-0305-9

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Experimental proof of the hypothesis on modulation of the transductor function of Na+,K+-ATPase by the ouabain-Ca2+ chelate complex was obtained by the method organotypic tissue culture. Quantum chemical estimations detected two principally different modes of Ca2+ ion chelation by ouabain molecule. It is hypothesized that the ouabain-Ca2+-Na+,K+-ATPase ligand-receptor complex is formed due to ion-ion bonds. The formation of the complex serves as the signal triggering the enzyme transductor function. It is experimentally proven that ouabain is incapable of inhibiting neurite growth in sensory neuron and heart tissue explants after removal of free calcium from the nutrient medium with EGTA.

引用

页码：436 / 438

页数：3

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