Limited oxygen conditions as an approach to scale-up and improvedandl-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineeredEscherichia coli

被引：8

作者：

Sierra-Ibarra, Estefania ^{[1
]}

Leal-Reyes, Laura J. ^{[1
]}

Huerta-Beristain, Gerardo ^{[1
,3
]}

Hernandez-Orihuela, Ana L. ^{[2
]}

Gosset, Guillermo ^{[1
]}

Martinez-Antonio, Agustino ^{[2
]}

Martinez, Alfredo ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Dept Ingn Celular & Biocatalisis, Inst Biotecnol, Ave Univ 2001, Cuernavaca 62210, Morelos, Mexico

[2] Inst Politecn Nacl, Dept Ingn Genet, Ctr Invest & Estudios Avanzados, Unidad Irapuato, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Guanajuato 36821, Mexico

[3] Univ Autonoma Guerrero, Fac Ciencias Quim Biol, Ave Lazaro Cardenas S-N Cd Univ, Chilpancingo 39070, Guerrero, Mexico

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2021年 / 44卷 / 02期

关键词：

Escherichia coli; Lactic acid; Scale-up; Avocado seed hydrolysate; Volumetric oxygen transfer coefficient; ENGINEERED ESCHERICHIA-COLI; D-LACTATE; HOMOFERMENTATIVE PRODUCTION; SUGARCANE BAGASSE; FERMENTATION; CORN; DEHYDROGENASE; GROWTH; STOVER;

D O I：

10.1007/s00449-020-02450-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effectiveness of micro-aeration on lactate (LA) production by metabolically engineeredEscherichia coliwas evaluated in 1 L bioreactors containing mineral media and glucose (70 g/L). Volumetric oxygen transfer coefficients (k(L)a) between 12.6 and 28.7 h(-1)increased the specific growth rate (mu) and volumetric productivity (Q(LA)) by 300 and 400%, respectively, without a significant decrease in lactate yield (Y-LA), when compared with non-aerated fermentations. A k(L)a of 12.6 h(-1)was successfully used as a criterion to scale-up the production oflandd-lactate from 1 to 11 and 130 L. Approximately constant Q(LA)and Y(LA)values were obtained throughout the fermentation scale-up process. Furthermore, ad-lactogenic fermentation was carried out in 1 L bioreactors using avocado seed hydrolysate as a culture medium under the same k(L)a value, displaying high Q(LA)and Y-LA.

引用

页码：379 / 389

页数：11

共 1 条

[1] Limited oxygen conditions as an approach to scale-up and improve d and l-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli
Estefanía Sierra-Ibarra
Laura J. Leal-Reyes
Gerardo Huerta-Beristain
Ana L. Hernández-Orihuela
Guillermo Gosset
Agustino Martínez-Antonio
Alfredo Martinez
Bioprocess and Biosystems Engineering, 2021, 44 : 379 - 389

← 1 →