Molecular Analysis of Staphylococcus epidermidis Strains Isolated from Community and Hospital Environments in China

被引:84
作者
Du, Xin [1 ]
Zhu, Yuanjun [1 ]
Song, Yan [1 ]
Li, Tianming [1 ]
Luo, Tao [2 ,3 ]
Sun, Gang [2 ,3 ]
Yang, Chongguang [2 ,3 ]
Cao, Cuiming [4 ]
Lu, Yuan [1 ]
Li, Min [1 ]
机构
[1] Fudan Univ, Shanghai Med Coll, Huashan Hosp, Dept Lab Med, Shanghai 200433, Peoples R China
[2] Fudan Univ, Key Lab Med Mol Virol, Inst Biomed Sci, Shanghai 200433, Peoples R China
[3] Fudan Univ, Inst Med Microbiol, Shanghai 200433, Peoples R China
[4] Shandong Univ, Jinan Cent Hosp, Dept Lab Med, Jinan 250100, Shandong, Peoples R China
来源
PLOS ONE | 2013年 / 8卷 / 05期
基金
中国国家自然科学基金;
关键词
CATABOLIC MOBILE ELEMENT; COAGULASE-NEGATIVE STAPHYLOCOCCI; ACME-ARCA GENE; METHICILLIN-RESISTANT; ANTIMICROBIAL RESISTANCE; BIOFILM FORMATION; AUREUS; SEQUENCE; MEC; INFECTIONS;
D O I
10.1371/journal.pone.0062742
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Staphylococcus epidermidis is a common cause of nosocomial infections worldwide. This study analyzed the differences in genetic endowment and clonal lineages with pathogenesis and resistance traits of S. epidermidis isolates collected from community and hospital environments (patients and healthcare staff) of the same ecological niche, time period, and geographical location in China. Methodology/Principal Findings: Molecular epidemiology and population analysis showed that nasal colonization rates of S. epidermidis in the community of Shanghai area of China and in healthcare personnel were 44.8% (methicillin-resistant S. epidermidis, MRSE: 17.2%) and 61.3% (MRSE: 30.0%), respectively. 86.7% of clinical isolates were MRSE. Among the strains studied, 44 sequence types (STs) were identified with 91.7% belonging to clonal complex 2 (CC2). Only 40.8% isolates from patients were also found in healthy individuals. MRSE-ST2-SCCmecIII was the predominant clone in clinical isolates, almost resistant to all antibiotics tested. Biofilm-related genes IS256 and icaA were detected in majority of the predominant clinical MRSE-ST2 clone with a 40.5% biofilm-positive rate. No ST2 isolate was found in community setting. We found a high prevalence of arginine catabolic mobile element (ACME) (74.1%). The prevalence of ACME-arc and ACME-opp3 clusters was 71.6% and 32.4%, respectively. Methicillin-sensitive S. epidermidis (MSSE) isolates harbored more ACME (83.3%) than MRSE isolates (67.7%), and there was no association between ACME and SCCmec types. An association was found between low-level ACME presence and invasive infections. Conclusions/Significance: We observed a high level of diversity within S. epidermidis in this study, with CC2 as the dominant clonal complex in both community and hospital settings. Only 40.8% of the isolates from patients were also found in healthy individuals. Contrary to that biofilm formation and multiple antibiotic resistance were associated closely with pathogenicity of S. epidermidis, ACME was more likely to be an indicator for colonization rather than a virulence factor.
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页数:10
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