Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti

被引：44

作者：

Costa-da-Silva, Andre Luis ^{[1
]}

Navarrete, Flavia Rosa ^{[1
]}

Salvador, Felipe Scassi ^{[2
]}

Karina-Costa, Maria ^{[1
]}

Ioshino, Rafaella Sayuri ^{[1
]}

Azevedo, Diego Soares ^{[1
]}

Rocha, Desiree Rafaela ^{[1
]}

Romano, Camila Malta ^{[2
]}

Capurro, Margareth Lara ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil

[2] Univ Sao Paulo, Virol Lab, Inst Trop Med, Sao Paulo, Brazil

来源：

PLOS ONE | 2013年 / 8卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

RHODNIUS-PROLIXUS; SYSTEM; INSECT;

D O I：

10.1371/journal.pone.0053816

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated "Glytube" was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti.

引用

页数：5