Resolving Neurodevelopmental and Vision Disorders Using Organoid Single-Cell Multi-omics

被引：21

作者：

Brancati, Giovanna ^{[1
,3
]}

Treutlein, Barbara ^{[3
]}

Camp, J. Gray ^{[1
,2
]}

机构：

[1] Inst Mol & Clin Ophthalmol, CH-4031 Basel, Switzerland

[2] Univ Basel, Dept Ophthalmol, CH-4031 Basel, Switzerland

[3] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland

来源：

NEURON | 2020年 / 107卷 / 06期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

PLURIPOTENT STEM-CELLS; HUMAN BRAIN-DEVELOPMENT; CEREBRAL ORGANOIDS; SPATIAL TRANSCRIPTOMICS; SELF-ORGANIZATION; GENETIC-VARIATION; DIFFERENTIATION; MODEL; LINES; MECHANISMS;

D O I：

10.1016/j.neuron.2020.09.001

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Human organoid models of the central nervous system, including the neural retina, are providing unprecedented opportunities to explore human neurodevelopment and neurodegeneration in controlled culture environments. In this Perspective, we discuss how the single-cell multi-omic toolkit has been used to identify features and limitations of brain and retina organoids and how these tools can be deployed to study congenital brain malformations and vision disorders in organoids. We also address how to improve brain and retina organoid protocols to revolutionize in vitro disease modeling.

引用

页码：1000 / 1013

页数：14

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