Resolving Neurodevelopmental and Vision Disorders Using Organoid Single-Cell Multi-omics

被引：21

作者：

Brancati, Giovanna ^{[1
,3
]}

Treutlein, Barbara ^{[3
]}

Camp, J. Gray ^{[1
,2
]}

机构：

[1] Inst Mol & Clin Ophthalmol, CH-4031 Basel, Switzerland

[2] Univ Basel, Dept Ophthalmol, CH-4031 Basel, Switzerland

[3] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland

来源：

NEURON | 2020年 / 107卷 / 06期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

PLURIPOTENT STEM-CELLS; HUMAN BRAIN-DEVELOPMENT; CEREBRAL ORGANOIDS; SPATIAL TRANSCRIPTOMICS; SELF-ORGANIZATION; GENETIC-VARIATION; DIFFERENTIATION; MODEL; LINES; MECHANISMS;

D O I：

10.1016/j.neuron.2020.09.001

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Human organoid models of the central nervous system, including the neural retina, are providing unprecedented opportunities to explore human neurodevelopment and neurodegeneration in controlled culture environments. In this Perspective, we discuss how the single-cell multi-omic toolkit has been used to identify features and limitations of brain and retina organoids and how these tools can be deployed to study congenital brain malformations and vision disorders in organoids. We also address how to improve brain and retina organoid protocols to revolutionize in vitro disease modeling.

引用

页码：1000 / 1013

页数：14

共 121 条

[1] Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform [J].

Achberger, Kevin ;

Probst, Christopher ;

Haderspeck, Jasmin ;

Bolz, Sylvia ;

Rogal, Julia ;

Chuchuy, Johanna ;

Nikolova, Marina ;

Cora, Virginia ;

Antkowiak, Lena ;

Haq, Wadood ;

Shen, Nian ;

Schenke-Layland, Katja ;

Ueffing, Marius ;

Liebau, Stefan ;

Loskill, Peter .

ELIFE, 2019, 8

[2] A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response [J].

Adamson, Britt ;

Norman, Thomas M. ;

Jost, Marco ;

Cho, Min Y. ;

Nunez, James K. ;

Chen, Yuwen ;

Villalta, Jacqueline E. ;

Gilbert, Luke A. ;

Horlbeck, Max A. ;

Hein, Marco Y. ;

Pak, Ryan A. ;

Gray, Andrew N. ;

Gross, Carol A. ;

Dixit, Atray ;

Parnas, Oren ;

Regev, Aviv ;

Weissman, Jonathan S. .

CELL, 2016, 167 (07) :1867-+

[3] First CRISPR therapy dosed [J].

不详 .

NATURE BIOTECHNOLOGY, 2020, 38 (04) :382-382

[4] FDA approves hereditary blindness gene therapy [J].

不详 .

NATURE BIOTECHNOLOGY, 2018, 36 (01) :6-6

[5] Control of human embryonic stem cell colony and aggregate size heterogeneity influences differentiation trajectories [J].

Bauwens, Celine Liu ;

Peerani, Raheem ;

Niebruegge, Sylvia ;

Woodhouse, Kimberly A. ;

Kumacheva, Eugenia ;

Husain, Mansoor ;

Zandstra, Peter W. .

STEM CELLS, 2008, 26 (09) :2300-2310

[6] Brains, Genes, and Primates [J].

Belmonte, Juan Carlos Izpisua ;

Callaway, Edward M. ;

Churchland, Patricia ;

Caddick, Sarah J. ;

Feng, Guoping ;

Homanics, Gregg E. ;

Lee, Kuo-Fen ;

Leopold, David A. ;

Miller, Cory T. ;

Mitchell, Jude F. ;

Mitalipov, Shoukhrat ;

Moutri, Alysson R. ;

Movshon, J. Anthony ;

Okano, Hideyuki ;

Reynolds, John H. ;

Ringach, Dario ;

Sejnowski, Terrence J. ;

Silva, Afonso C. ;

Strick, Peter L. ;

Wu, Jun ;

Zhang, Feng .

NEURON, 2015, 86 (03) :617-631

[7] Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia [J].

Bershteyn, Marina ;

Nowakowski, Tomasz J. ;

Pollen, Alex A. ;

Di Lullo, Elizabeth ;

Nene, Aishwarya ;

Wynshaw-Boris, Anthony ;

Kriegstein, Arnold R. .

CELL STEM CELL, 2017, 20 (04) :435-+

[8] Cell stress in cortical organoids impairs molecular subtype specification [J].

Bhaduri, Aparna ;

Andrews, Madeline G. ;

Mancia Leon, Walter ;

Jung, Diane ;

Shin, David ;

Allen, Denise ;

Jung, Dana ;

Schmunk, Galina ;

Haeussler, Maximilian ;

Salma, Jahan ;

Pollen, Alex A. ;

Nowakowski, Tomasz J. ;

Kriegstein, Arnold R. .

NATURE, 2020, 578 (7793) :142-+

[9] Assembly of functionally integrated human forebrain spheroids [J].

Birey, Fikri ;

Andersen, Jimena ;

Makinson, Christopher D. ;

Islam, Saiful ;

Wei, Wu ;

Huber, Nina ;

Fan, H. Christina ;

Metzler, Kimberly R. Cordes ;

Panagiotakos, Georgia ;

Thom, Nicholas ;

O'Rourke, Nancy A. ;

Steinmetz, Lars M. ;

Bernstein, Jonathan A. ;

Hallmayer, Joachim ;

Huguenard, John R. ;

Pasca, Sergiu P. .

NATURE, 2017, 545 (7652) :54-+

[10]

Bock C., 2020, ZENODO, DOI [10.5281/zenodo.4001718, DOI 10.5281/ZENODO.4001718]

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