Influence of temperature, time and different media on mesenchymal stromal cells shipped for clinical application

被引：22

作者：

Bronzini, I. ^{[1
]}

Patruno, M. ^{[1
]}

Iacopetti, I. ^{[2
]}

Martinello, T. ^{[1
]}

机构：

[1] Univ Padua, Dept Comparat Biomed & Food Sci, I-35100 Padua, Italy

[2] Univ Padua, Dept Anim Med Prod & Hlth, I-35100 Padua, Italy

来源：

VETERINARY JOURNAL | 2012年 / 194卷 / 01期

关键词：

Equine; Canine; Mesenchymal stromal cells; Shipping; Senescence; CRYOPRESERVATION;

D O I：

10.1016/j.tvjl.2012.03.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Cell-based therapies, such as the use of mesenchymal stromal cells (MSCs), are becoming popular in veterinary medicine. When MSCs are not cryopreserved, they are shipped in suspension, but no previous studies have analyzed MSC viability during delivery. Here, the impact of several experimental shipping conditions on the number of equine blood-derived (ePB-MSC) and canine adipose-derived (cA-MSC) MSCs were evaluated. Among the different parameters tested, only time and temperature influenced MSC number during the experimental shipping conditions. Cells were monitored over different time intervals for gene expression of typical MSC markers and to evaluate acquired resistance to apoptosis and beta-galactosidase activity. Overall, these results indicate that ePB-MSC and cA-MSC should be delivered in phosphate buffered saline at room temperature and within 9-12 h. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：121 / 123

页数：3

共 10 条

[1] Cellular senescence: when bad things happen to good cells [J].

Campisi, Judith ;

di Fagagna, Fabrizio d'Adda .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (09) :729-740

[2] A BIOMARKER THAT IDENTIFIES SENESCENT HUMAN-CELLS IN CULTURE AND IN AGING SKIN IN-VIVO [J].

DIMRI, GP ;

LEE, XH ;

BASILE, G ;

ACOSTA, M ;

SCOTT, C ;

ROSKELLEY, C ;

MEDRANO, EE ;

LINSKENS, M ;

RUBELJ, I ;

PEREIRASMITH, O ;

PEACOCKE, M ;

CAMPISI, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (20) :9363-9367

[3] New surgical treatments for osteochondritis dissecans and subchondral bone cysts [J].

Fortier, LA ;

Nixon, AJ .

VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE, 2005, 21 (03) :673-+

[4]

Haack-Sorensen M, 2011, METHODS MOL BIOL, V698, P161, DOI 10.1007/978-1-60761-999-4_13

[5]

Koch TG, 2009, CAN VET J, V50, P155

[6]

KOZHEVNIKOVA MN, 2008, IZV AKAD NAUK BIOL+, V2, P156

[7] Canine adipose-derived-mesenchymal stem cells do not lose stem features after a long-term cryopreservation [J].

Martinello, T. ;

Bronzini, I. ;

Maccatrozzo, L. ;

Mollo, A. ;

Sampaolesi, M. ;

Mascarello, F. ;

Decaminada, M. ;

Patruno, M. .

RESEARCH IN VETERINARY SCIENCE, 2011, 91 (01) :18-24

[8]

Martinello T, 2010, TISSUE ENG PART C-ME, V16, P771, DOI [10.1089/ten.tec.2009.0512, 10.1089/ten.TEC.2009.0512]

[9] Temporal Analysis of Equine Bone Marrow Aspirate During Establishment of Putative Mesenchymal Progenitor Cell Populations [J].

Radcliffe, Catherine H. ;

Flaminio, M. Julia B. F. ;

Fortier, Lisa A. .

STEM CELLS AND DEVELOPMENT, 2010, 19 (02) :269-281

[10] How to track cellular aging of mesenchymal stromal cells? [J].

Wagner, Wolfgang ;

Bork, Simone ;

Lepperdinger, Guenther ;

Joussen, Sylvia ;

Ma, Nan ;

Strunk, Dirk ;

Koch, Carmen .

AGING-US, 2010, 2 (04) :224-230

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