Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses

被引：4

作者：

Cong, Feng ^{[1
]}

Zhu, Yujun ^{[1
]}

Liu, Xiangnan ^{[1
,3
]}

Li, Xiuzhen ^{[1
]}

Chen, Meili ^{[2
]}

Huang, Ren ^{[2
]}

Guo, Pengju ^{[1
]}

机构：

[1] Guangdong Lab Anim Monitoring Inst, Guangzhou 5106, Guangdong, Peoples R China

[2] Guangdong Prov Key Lab Lab Anim, Guangzhou 5106, Guangdong, Peoples R China

[3] South China Agr Univ, Coll Vet Med, Guangzhou, Guangdong, Peoples R China

来源：

MOLECULAR AND CELLULAR PROBES | 2018年 / 37卷

关键词：

Luminex; xTAG-multiplex PCR; AIV; NDV; IBV; ILTV; POLYMERASE-CHAIN-REACTION; PATHOGENS; ASSAY;

D O I：

10.1016/j.mcp.2017.10.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 x 10(2) -3.52 x 10(3) copies/mu L. We examined 60 clinical specimens and found 18 positive for respiratory viruses. Our result demonstrated that xTAG-multiplex PCR method is a high-throughput, rapid, specific and sensitive assay for use in epidemiological studies and clinical detection of avian respiratory pathogens.

引用

页码：1 / 5

页数：5

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