Simultaneous determination of multi-residue and multi-class antibiotics in aquaculture shrimps by UPLC-MS/MS

被引：58

作者：

Saxena, Sushil Kumar ^{[1
]}

Rangasamy, Rajesh ^{[2
]}

Krishnan, Anoop A. ^{[2
]}

Singh, Dhirendra P. ^{[2
]}

Uke, Sumedh P. ^{[2
]}

Malekadi, Praveen Kumar ^{[2
]}

Sengar, Anoop S. ^{[3
]}

Mohamed, D. Peer ^{[2
]}

Gupta, Ananda ^{[4
]}

机构：

[1] Export Inspect Council, 3rd Floor,NDYMCA Cultural Ctr Bldg 1,Jai Singh Rd, New Delhi 110001, India

[2] Export Inspect Agcy Kolkata Lab, 101 Southend Conclave,1582 Rajdanga Main Rd, Kolkata 700107, India

[3] Export Inspect Agcy Mumbai, MIDC, Pilot Test House,E-3, Bombay, Maharashtra, India

[4] Export Inspect Agcy Kolkata, World Trade Ctr, 14-1B,Ezra St, Kolkata 700001, India

来源：

FOOD CHEMISTRY | 2018年 / 260卷

关键词：

Multi-class multi-residue; Antibiotics; Aquaculture products; UPLC-MS/MS; Validation; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; MULTIRESIDUE DETERMINATION; VETERINARY DRUGS; FLUOROQUINOLONE ANTIBIOTICS; QUALITY-ASSURANCE; SALMON MUSCLE; BOVINE MUSCLE; FISH SAMPLES; SULFONAMIDES;

D O I：

10.1016/j.foodchem.2018.04.018

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An accurate, reliable and fast multi-residue, multi-class method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed and validated for simultaneous determination and quantification of 24 pharmacologically active substances of three different classes (Quinolones including fluoroquinolones, sulphonamides and tetracyclines) in aquaculture shrimps. Sample preparation involves extraction with acetonitrile containing 0.1% formic acid and followed by clean up with n-hexane and 0.1% methanol in water by UPLC-MS/MS within 8 min. The method was validated according to European Commission Decision 2002/657. Acceptable values were obtained for linearity (5-200 mu g kg(-1)), specificity, Limit of Quantification (5-10 mu g kg(-1)), recovery (between 83 and 100%), repeatability (RSD < 9%), within lab reproducibility (RSD < 15%), reproducibility (RSD <= 22%), decision limit (105-116 mu g kg(-1)) and detection capability (110-132 mu g kg(-1)). The validated method was applied to aquaculture shrimp samples from India.

引用

页码：336 / 343

页数：8