Regulation of mammalian nitric oxide synthases by electrostatic interactions in the linker region of calmodulin

Calmodulin (CaM), the ubiquitous Ca2+-sensing protein, consists of two globular domains separated by a flexible central linker that properly orients CaM's globular domains to bind and regulate various intracellular proteins, including the nitric oxide synthase (NOS) enzymes. In the present study we determined that the charge and length of the central linker of CaM has an effect on the binding and activation of the NOS isozymes by using a variety of charge CaM mutants (T79D, S81D, T79D/581 D, 5101 D and E84R/E87K) and CaM mutants with residues removed (Delta 84, Delta 83-84, and Delta 81-84). Our kinetic and spectropolarimetry, results demonstrate that the NOS enzymes are not adversely affected by the CaM mutants with the exceptions of S101D, E84R/E87K and the deletion of residue 84. Electrostatic interactions in the central linker between residues 82-87 in combination with hydrophobic interactions in the globular domains of CaM are important for its tight association to inducible NOS. (c) 2008 Elsevier B.V. All rights reserved.