Different functions of PHF10 isoforms - subunits of the PBAF chromatin remodeling complex

被引:3
作者
Sheynov, A. A. [1 ]
Tatarskiy, V. V. [1 ]
Azieva, A. M. [1 ,2 ]
Georgieva, S. G. [1 ]
Soshnikova, N., V [1 ]
机构
[1] RAS, Dept Eukariot Transcript Factors, Inst Gene Biol, Moscow, Russia
[2] Natl Res Ctr, Kurchatov Inst, Moscow, Russia
来源
VAVILOVSKII ZHURNAL GENETIKI I SELEKTSII | 2019年 / 23卷 / 02期
基金
俄罗斯科学基金会;
关键词
chromatin remodeling; PBAF complex; PHF10; isoforms; SWI/SNF; PHOSPHORYLATION; PATHWAYS;
D O I
10.18699/VJ19.480
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Chromatin remodelling multiprotein complexes play an important role in regulation of gene expression in embryogenesis and in the adult organism. Mutations in the subunits of the complexes are often lethal or lead to developmental defects. Complexes consist of core subunits and a specific module. The core consists of ATPase and structure subunits, specific subunits of the module are necessary for chromatin binding. PHF10 (PHD finger protein 10) is a subunit of the PBAF (polybromo-associated BAF) chromatin remodelling complex subfamily. Conserved and highly regulated PHF10 is ubiquitously expressed in mammals as four different isoforms. The isoforms of PHF10 differ by domain structures and posttranslational modifications. All isoforms are highly regulated and included in the PBAF complex in a mutually exclusive manner.Two of the PHF10 isoforms (PHF10-P) are expressed at a high level in neuronal and myeloid progenitors and are necessary for cell proliferation. These isoforms contain PHD (plant homeodomain) fingers for nucleosome binding and recruit RNA polymerase II on the promoters of cell cycle genes.Two other isoforms (PI-IF10-S) instead of PHD have PDSM (phosphorylation-dependent sumoylation motif), the motif for SUMO1 conjugation. PHF 10 is the most unstable subunit of the PBAF complex. Stability can alter the turnover rate of the subunits of the PBAF complex. All PHF10 isoforms are degraded by beta-TrCP ubiquitin ligase but PHF10-S isoforms contain a cluster of serins (X-cluster) for multiple phosphorylation by casein kinase I. This phosphorylation protects the beta-TrCP degron from beta-TrCP recognition and subsequently stabilizes the PHF10-S isoforms. Thus, the incorporation of PHF10 isoforms with different phosphorylation patterns and different stability into the PBAF complexes alters the functions of the entire PBAF complex and determines the range of genes undergoing remodelling.
引用
收藏
页码:184 / 189
页数:6
相关论文
共 21 条
[1]   The SWI/SNF Subunit INI1 Contains an N-Terminal Winged Helix DNA Binding Domain that Is a Target for Mutations in Schwannomatosis [J].
Allen, Mark D. ;
Freund, Stefan M. V. ;
Zinzalla, Giovanna ;
Bycroft, Mark .
STRUCTURE, 2015, 23 (07) :1344-1349
[2]   Stability of Chromatin Remodeling Complex Subunits Is Determined by Their Phosphorylation Status [J].
Azieva, A. M. ;
Sheinov, A. A. ;
Galkin, F. A. ;
Georgieva, S. G. ;
Soshnikova, N. V. .
DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2018, 479 (01) :66-68
[3]   PHF10 Isoforms Are Phosphorylated in the PBAF Mammalian Chromatin Remodeling Complex [J].
Brechalov, A. V. ;
Valieva, M. E. ;
Georgieva, S. G. ;
Soshnikova, N. V. .
MOLECULAR BIOLOGY, 2016, 50 (02) :278-283
[4]   Mammalian cells contain two functionally distinct PBAF complexes incorporating different isoforms of PHF10 signature subunit [J].
Brechalov, Alexander V. ;
Georgieva, Sofia G. ;
Soshnikova, Nataliya V. .
CELL CYCLE, 2014, 13 (12) :1970-1979
[5]   Phosphorylation of the chromatin remodeling factor DPF3a induces cardiac hypertrophy through releasing HEY repressors from DNA [J].
Cui, Huanhuan ;
Schlesinger, Jenny ;
Schoenhals, Sophia ;
Toenjes, Martje ;
Dunkel, Ilona ;
Meierhofer, David ;
Cano, Elena ;
Schulz, Kerstin ;
Berger, Michael F. ;
Haack, Timm ;
Abdelilah-Seyfried, Salim ;
Bulyk, Martha L. ;
Sauer, Sascha ;
Sperling, Silke R. .
NUCLEIC ACIDS RESEARCH, 2016, 44 (06) :2538-2553
[6]  
FlyBase, FLYBASE GEN REP DM E
[7]   Chromatin remodelling during development [J].
Ho, Lena ;
Crabtree, Gerald R. .
NATURE, 2010, 463 (7280) :474-484
[8]   The Many Roles of BAF (mSWI/SNF) and PBAF Complexes in Cancer [J].
Hodges, Courtney ;
Kirkland, Jacob G. ;
Crabtree, Gerald R. .
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2016, 6 (08)
[9]   Writing, erasing and reading histone lysine methylations [J].
Hyun, Kwangbeom ;
Jeon, Jongcheol ;
Park, Kihyun ;
Kim, Jaehoon .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2017, 49 :e324-e324
[10]   The BAF45a/PHF10 subunit of SWI/SNF-like chromatin remodeling complexes is essential for hematopoietic stem cell maintenance [J].
Krasteva, Veneta ;
Crabtree, Gerald R. ;
Lessard, Julie A. .
EXPERIMENTAL HEMATOLOGY, 2017, 48 :58-71