Increased apoptosis is specific for acute rejection in rat small bowel transplant

被引：27

作者：

Fishbein, TM ^{[1
]}

Liu, JH ^{[1
]}

Wang, LQ ^{[1
]}

Li, YS ^{[1
]}

Boros, P ^{[1
]}

机构：

[1] CUNY Mt Sinai Sch Med, Recanati Miller Transplantat Inst, New York, NY 10029 USA

来源：

JOURNAL OF SURGICAL RESEARCH | 2004年 / 119卷 / 01期

关键词：

apoptosis; intestinal transplantation; ischemia-reperfusion injury; rejection;

D O I：

10.1016/j.jss.2003.12.028

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Apoptosis has been associated with several events in solid organ transplantation, including ischemia/ reperfusion (IR) injury and acute rejection. To determine whether apoptosis-profiles may distinguish these two conditions, we analyzed apoptosis rates in a rat orthotopic small bowel transplant (SBT) model. SBT was performed in Lewis rats with either freshly harvested or preserved (4 h, in UW at 4degreesC) syngeneic and allogeneic(Brown-Norway) grafts. Bowel samples were collected 2 h after reperfusion and on small bowel transplant postoperative days (POD) 1, 4, and 7. Apoptosis was detected by measuring levels of histone-associated DNA fragments and caspase 3 expression, and by determining apoptotic body counts. All markers measured 2 h after reperfusion increased profoundly in association with preservation. After a significant decrease on POD 1, apoptosis rates rose again between POD 4 and 7 only in allogeneic grafts. This distinct second increase in apoptosis may be an early and specific sign of acute rejection. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：51 / 55

页数：5