Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope

被引：3
|
作者：
Zheng, ZW
Yang, PH [1 ]
Zeng, GC
Cai, JY
机构：
[1] S China Univ Technol, Minist Educ, Key Lab Specially Funct Mat & Adv Mfg Technol, Guangzhou 510640, Peoples R China
[2] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2006年 / 51卷 / 04期
基金：
中国国家自然科学基金;
关键词：
holo-transferrin; apo-transferrin; anti-transferrin; AFM;
D O I：
10.1007/s11434-006-0405-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also characterized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-transferrin more tightly than apo-transferrin. The force-distance curves revealed that the affinity of anti-transferrin and holo-transferrin was much stronger than that of apo-transferrin.
引用
收藏
页码：405 / 408
页数：4
相关论文
共 50 条
  • [41] INTERACTION BETWEEN TRANSFERRIN AND RABBIT RETICULOCYTE GHOSTS
    MORGAN, EH
    BAKER, E
    BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 363 (02) : 240 - 248
  • [42] KINETICS OF INTERACTION BETWEEN RABBIT TRANSFERRIN AND RETICULOCYTES
    BAKER, E
    MORGAN, EH
    BIOCHEMISTRY, 1969, 8 (03) : 1133 - &
  • [43] Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation
    Tillotson, Benjamin J.
    Goulatis, Loukas I.
    Parenti, Isabelle
    Duxbury, Elizabeth
    Shusta, Eric V.
    PLOS ONE, 2015, 10 (12):
  • [44] Systemic p53 Gene Therapy of Cancer with Immunolipoplexes Targeted by Anti-Transferrin Receptor scFv
    Liang Xu
    Wen-Hua Tang
    Cheng-Cheng Huang
    William Alexander
    Lai-Man Xiang
    Kathleen F. Pirollo
    Antonina Rait
    Esther H. Chang
    Molecular Medicine, 2001, 7 : 723 - 734
  • [45] An anti-transferrin receptor antibody enhanced the growth inhibitory effects of chemotherapeutic drugs on human glioma cells
    Xu, Guozheng
    Wen, Xue
    Hong, Yi
    Du, Hao
    Zhang, Xinyuan
    Song, Jian
    Yin, Yimei
    Huang, He
    Shen, Guanxin
    INTERNATIONAL IMMUNOPHARMACOLOGY, 2011, 11 (11) : 1844 - 1849
  • [46] Probing the machinery of intracellular trafficking with the atomic force microscope
    Kumar, S
    Hoh, JH
    TRAFFIC, 2001, 2 (11) : 746 - 756
  • [47] PROBING NANOSCALE FORCES WITH THE ATOMIC-FORCE MICROSCOPE
    PRATER, C
    MAIVALD, P
    KJOLLER, K
    HEATON, M
    R&D MAGAZINE, 1995, 37 (09): : 63 - 64
  • [48] Nanomechanical probing of microbubbles using the atomic force microscope
    Sboros, V.
    Glynos, E.
    Pye, S. D.
    Moran, C. M.
    Butler, M.
    Ross, J. A.
    McDicken, W. N.
    Koutsos, V.
    ULTRASONICS, 2007, 46 (04) : 349 - 354
  • [49] Systemic p53 gene therapy of cancer with immunolipoplexes targeted by anti-transferrin receptor scFv
    Xu, L
    Tang, WH
    Huang, CC
    Alexander, W
    Xiang, LM
    Pirollo, KF
    Rait, A
    Chang, EH
    MOLECULAR MEDICINE, 2001, 7 (10) : 723 - 734
  • [50] ANTITUMOR-ACTIVITY IN MICE OF AN IMMUNOTOXIN MADE WITH ANTI-TRANSFERRIN RECEPTOR AND A RECOMBINANT FORM OF PSEUDOMONAS EXOTOXIN
    BATRA, JK
    JINNO, Y
    CHAUDHARY, VK
    KONDO, T
    WILLINGHAM, MC
    FITZGERALD, DJ
    PASTAN, I
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (21) : 8545 - 8549
12345