Probing molecular interaction between transferrin and anti-transferrin by atomic force microscope

被引：3
|
作者：
Zheng, ZW
Yang, PH [1 ]
Zeng, GC
Cai, JY
机构：
[1] S China Univ Technol, Minist Educ, Key Lab Specially Funct Mat & Adv Mfg Technol, Guangzhou 510640, Peoples R China
[2] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2006年 / 51卷 / 04期
基金：
中国国家自然科学基金;
关键词：
holo-transferrin; apo-transferrin; anti-transferrin; AFM;
D O I：
10.1007/s11434-006-0405-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interaction between transferrin (Tf) and its antibody was investigated by atomic force microscope. Tf-antibody was immobilized on the Au-coated glass slide, and the specific combination between antibody and antigen was also characterized by AFM. The results showed that holo-transferrin was jogged with anti-transferrin, and binded anti-transferrin more tightly than apo-transferrin. The force-distance curves revealed that the affinity of anti-transferrin and holo-transferrin was much stronger than that of apo-transferrin.
引用
收藏
页码：405 / 408
页数：4
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