Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs

被引：15
作者：
Herbst, Werner [1 ]
Willems, Hermann [2 ]
机构：
[1] Justus Liebig Univ, Inst Hyg & Infect Dis Anim, Frankfurter Str 89, D-35392 Giessen, Germany
[2] Justus Liebig Univ, Clin Ruminants & Swine, Frankfurter Str 112, D-35392 Giessen, Germany
来源：
RESEARCH IN VETERINARY SCIENCE | 2017年 / 115卷
关键词：
Dog; Feces; Electron microscopy; Circovirus; TYPE-2; PCV2; DISEASES; TISSUES; PROTEIN; CATTLE; PIGS;
D O I：
10.1016/j.rvsc.2017.01.014
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768 bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码：51 / 53
页数：3
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