Lactobacillus sakeisuppresses collagen-induced arthritis and modulates the differentiation of T helper 17 cells and regulatory B cells

被引：26

作者：

Jhun, Jooyeon ^{[1
,2
]}

Min, Hong Ki ^{[3
]}

Ryu, Jaeyoon ^{[1
,2
]}

Lee, Seon-Yeong ^{[1
,2
]}

Ryu, Jun-Geol ^{[1
,2
]}

Choi, Jeong Won ^{[1
,2
]}

Na, Hyun Sik ^{[1
,2
]}

Lee, Seung Yoon ^{[1
,2
]}

Jung, Yunju ^{[4
,5
]}

Park, Sang-Jun ^{[5
]}

Park, Myeong Soo ^{[4
]}

Kwon, Bin ^{[4
]}

Ji, Geun Eog ^{[4
,5
]}

Cho, Mi-La ^{[1
,2
,6
]}

Park, Sung-Hwan ^{[7
]}

机构：

[1] Catholic Univ Korea, Rheumatism Res Ctr, Catholic Res Inst Med Sci, Coll Med, Seoul 06591, South Korea

[2] Catholic Univ Korea, Coll Med, Lab Immune Network, Catholic Res Inst Med Sci, Seoul, South Korea

[3] Konkuk Univ, Dept Internal Med, Div Rheumatol, Med Ctr, Seoul, South Korea

[4] BIFIDO Co Ltd, Res Ctr, Hongcheon, Gangwon Do, South Korea

[5] Seoul Natl Univ, Res Inst Human Ecol, Dept Food & Nutr, Seoul, South Korea

[6] Catholic Univ Korea, Coll Med, Dept Med Lifesci, 222 Banpo Daero, Seoul 06591, South Korea

[7] Catholic Univ Korea, Coll Med, Dept Internal Med, Devis Rheumatol,Seoul St Marys Hosp, Seoul, South Korea

来源：

JOURNAL OF TRANSLATIONAL MEDICINE | 2020年 / 18卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Rheumatoid arthritis; Microbiome; Lactobacillus sakei; T helper 17 cell; Regulatory B cell; RHEUMATOID-ARTHRITIS; AUTOIMMUNE ARTHRITIS; TH17; CELLS; PORPHYROMONAS-GINGIVALIS; SAKEI; MICROBIOTA; IMMUNITY;

D O I：

10.1186/s12967-020-02477-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background To evaluate the immunomodulatory effect ofLactobacillus sakeiin a mouse model of rheumatoid arthritis (RA) and in human immune cells. Methods We evaluated whetherL. sakeireduced the severity of collagen-induced arthritis (CIA) and modulated interleukin (IL)-17 and IL-10 levels, as well as whether it affected the differentiation of CD4(+)T cells and regulatory B cells. We evaluated osteoclastogenesis after culturing bone marrow-derived mononuclear cells withL. sakei. Results The differentiation of T helper 17 cells and the serum level of IL-17 were suppressed byL. sakeiin both human peripheral blood mononuclear cells and mouse splenocytes. The serum level of IL-10 was significantly increased in theL. sakei-treated group, whereas the regulatory T cell population was unchanged. The population of regulatory B cells significantly increased the inL. sakei-treated group. Oral administration ofL. sakeireduced the arthritis incidence and score in mice with CIA. Finally, osteoclastogenesis and the mRNA levels of osteoclast-related genes were suppressed in theL. sakei-treated group. Conclusion L. sakeiexerted an anti-inflammatory effect in an animal model of RA, regulated Th17 and regulatory B cell differentiation, and suppressed osteoclastogenesis. Our findings suggest thatL. sakeihas therapeutic potential for RA.

引用

页数：11

共 39 条

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