Magnetite Nanoparticles for Stem Cell Labeling with High Efficiency and Long-Term in Vivo Tracking

被引：44

作者：

Guldris, Noelia ^{[1
,2
,3
]}

Argibay, Barbara ^{[3
]}

Gallo, Juan ^{[1
]}

Iglesias-Rey, Ramon ^{[3
]}

Carbo-Argibay, Enrique ^{[1
]}

Kolen'ko, Yury V. ^{[1
]}

Campos, Francisco ^{[3
]}

Sobrino, Tomas ^{[3
]}

Salonen, Laura M. ^{[1
]}

Banobre-Lopez, Manuel ^{[1
]}

Castillo, Jose ^{[3
]}

Rivas, Jose ^{[1
,2
]}

机构：

[1] Int Iberian Nanotechnol Lab, Ave Mestre Jose Veiga S-N, P-4715330 Braga, Portugal

[2] Univ Santiago de Compostela, Nanotechnol & Magnetism Lab, Technol Res Inst, Dept Appl Phys, Santiago De Compostela 15782, Spain

[3] Univ Santiago de Compostela, Clin Neurosci Res Lab, Clin Univ Hosp, Hlth Res Inst Santiago de Compostela IDIS, Santiago De Compostela 15782, Spain

来源：

BIOCONJUGATE CHEMISTRY | 2017年 / 28卷 / 02期

基金：

欧盟地平线“2020”;

关键词：

IRON-OXIDE NANOPARTICLES; CONTRAST AGENTS; MRI; THERAPY; STABILITY; OPTIMIZATION; SEPARATION; PARTICLES; CULTURE; IMPACT;

D O I：

10.1021/acs.bioconjchem.6b00522

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Superparamagnetic iron oxide nanoparticles (SPIO-PAA), ultrasmall iron oxide nanoparticles (USPIO-PAA), and glucosamine-modified iron oxide nanoparticles (USPIO-PAA-GIcN) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounced differences were found in the labeling performance of the three samples in terms of cellular dose and labeling efficiency. In combination with polylysine, SPIO-PAA showed nonhomogeneous cell internalization, while for USPIO-PAA no uptake was found. On the contrary, USPIO-PAA-GIcN featured high cellular uptake and biocompatibility, and sensitive detection in both in vitro and in vivo experiments was found by MRI, showing that glucosamine functionalization can be an efficient strategy to increase cell uptake of ultrasmall iron oxide nanoparticles by MSCs.

引用

页码：362 / 370

页数：9

共 44 条

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