The Epidermal Basement Membrane Is a Composite of Separate Laminin- or Collagen IV-containing Networks Connected by Aggregated Perlecan, but Not by Nidogens

被引:118
作者
Behrens, Daniel Timo [1 ]
Villone, Daniela [1 ]
Koch, Manuel [2 ]
Brunner, Georg [3 ]
Sorokin, Lydia [1 ]
Robenek, Horst [4 ]
Bruckner-Tuderman, Leena [5 ]
Bruckner, Peter [1 ]
Hansen, Uwe [1 ]
机构
[1] Munster Univ Hosp, Inst Physiol Chem & Pathobiochem, D-48149 Munster, Germany
[2] Univ Cologne, Ctr Biochem, Inst Dent Res & Musculoskeletal Biol, D-50931 Cologne, Germany
[3] Fachklin Hornheide, Dept Tumor Res, D-48157 Munster, Germany
[4] Leibniz Inst Arteriosclerosis Res, D-48149 Munster, Germany
[5] Univ Med Ctr, Dept Dermatol, D-79104 Freiburg, Germany
关键词
HEPARAN-SULFATE PROTEOGLYCAN; VII COLLAGEN; BINDING; PROTEIN; SKIN; CHAINS;
D O I
10.1074/jbc.M111.336073
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The basement membrane between the epidermis and the dermis is indispensable for normal skin functions. It connects, and functionally separates, the epidermis and the dermis. To understand the suprastructural and functional basis of these connections, heterotypic supramolecular aggregates were isolated from the dermal-epidermal junction zone of human skin. Individual suprastructures were separated and purified by immunomagnetic beads, each recognizing a specific, molecular component of the aggregates. The molecular compositions of the suprastructures were determined by immunogold electron microscopy and immunoblotting. A composite of two networks was obtained from fibril-free suspensions by immunobeads recognizing either laminin 332 or collagen IV. After removal of perlecan-containing suprastructures or after enzyme digestion of heparan sulfate chains, a distinct network with a diffuse electron-optical appearance was isolated with magnetic beads coated with antibodies to collagen IV. The second network was more finely grained and comprised laminin 332 and laminins with alpha 5-chains. The core protein of perlecan was an exclusive component of this network whereas its heparan sulfate chains were integrated into the collagen IV-containing network. Nidogens 1 and 2 occurred in both networks but did not form strong molecular cross-bridges. Their incorporation into one network appeared to be masked after their incorporation into the other one. We conclude that the epidermal basement membrane is a composite of two structurally independent networks that are tightly connected in a spot-welding-like manner by perlecan-containing aggregates.
引用
收藏
页码:18700 / 18709
页数:10
相关论文
共 28 条
[11]   The anchorless adhesin Eap (extracellular adherence protein) from Staphylococcus aureus selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules [J].
Hansen, U ;
Hussain, M ;
Villone, D ;
Herrmann, M ;
Robenek, H ;
Peters, G ;
Sinha, B ;
Bruckner, P .
MATRIX BIOLOGY, 2006, 25 (04) :252-260
[12]   The distributions of type IV collagen α chains in basement membranes of human epidermis and skin appendages [J].
Hasegawa, Haruko ;
Naito, Ichiro ;
Nakano, Kazuyo ;
Momota, Ryusuke ;
Nishida, Keiichiro ;
Taguchi, Takehito ;
Sado, Yoshikazu ;
Ninomiya, Yoshifumi ;
Ohtsuka, Aiji .
ARCHIVES OF HISTOLOGY AND CYTOLOGY, 2007, 70 (04) :255-265
[13]   Nidogens -: Extracellular matrix linker molecules [J].
Ho, Matthew S. P. ;
Boese, Kerstin ;
Mokkapati, Sharada ;
Nischt, Roswitha ;
Smyth, Neil .
MICROSCOPY RESEARCH AND TECHNIQUE, 2008, 71 (05) :387-395
[14]   Alport's syndrome, Goodpasture's syndrome, and type IV collagen [J].
Hudson, BG ;
Tryggvason, K ;
Sundaramoorthy, M ;
Neilson, EG .
NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (25) :2543-2556
[15]   Discrete integration of collagen XVI into tissue-specific collagen fibrils or beaded microfibrils [J].
Kassner, A ;
Hansen, U ;
Miosge, N ;
Reinhardt, DP ;
Aigner, T ;
Bruckner-Tuderman, L ;
Bruckner, P ;
Grässel, S .
MATRIX BIOLOGY, 2003, 22 (02) :131-143
[16]   TYPE-VII COLLAGEN FORMS AN EXTENDED NETWORK OF ANCHORING FIBRILS [J].
KEENE, DR ;
SAKAI, LY ;
LUNSTRUM, GP ;
MORRIS, NP ;
BURGESON, RE .
JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :611-621
[17]   Mammalian collagen IV [J].
Khoshnoodi, Jamshid ;
Pedchenko, Vadim ;
Hudson, Billy G. .
MICROSCOPY RESEARCH AND TECHNIQUE, 2008, 71 (05) :357-370
[18]  
MARINKOVICH MP, 1992, J BIOL CHEM, V267, P17900
[19]   BINDING-PROPERTIES AND PROTEASE STABILITY OF RECOMBINANT HUMAN NIDOGEN [J].
MAYER, U ;
ZIMMERMANN, K ;
MANN, K ;
REINHARDT, D ;
TIMPL, R ;
NISCHT, R .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 227 (03) :681-686
[20]   Structural and genetic analysis of laminin-nidogen interaction [J].
Mayer, U ;
Kohfeldt, E ;
Timpl, R .
MORPHOGENESIS: CELLULAR INTERACTIONS, 1998, 857 :130-142