A study on mutual interaction between cytokine induced killer cells Italy and umbilical cord-derived mesenchymal cells: Implication for their in-vivo use

被引：10

作者：

Chieregato, Katia ^{[1
]}

Albiero, Elena ^{[1
,2
]}

Castegnaro, Silvia ^{[1
]}

Bernardi, Martina ^{[1
,2
]}

d'Amore, Emanuele S. G. ^{[3
]}

Zanon, Cristina ^{[1
]}

Madeo, Domenico ^{[2
]}

Rodeghiero, Francesco ^{[1
,2
]}

Astori, Giuseppe ^{[1
]}

机构：

[1] San Bortolo Hosp, Adv Cellular Therapy Lab, Dept Cellular Therapy & Hematol, I-36100 Vicenza, Italy

[2] Hematol Project Fdn, Vicenza, Italy

[3] San Bortolo Hosp, Dept Pathol, I-36100 Vicenza, Italy

来源：

BLOOD CELLS MOLECULES AND DISEASES | 2012年 / 49卷 / 3-4期

关键词：

Cytokine-induced-killer (CIK) cells; Mesenchymal stromal cells (MSC); Cytotoxicity; Co-culture; Umbilical cord; HUMAN ADIPOSE-TISSUE; STEM-CELLS; BONE-MARROW; ADOPTIVE IMMUNOTHERAPY; STROMAL CELLS; GASTRIC-CANCER; RICH SOURCE; CIK CELLS; GRAFT; THERAPY;

D O I：

10.1016/j.bcmd.2012.05.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Recently a number of cellular therapy based-clinical trials have been carried out using mesenchymal stromal cells (MSC) or cytokine-induced-killer (CIK) cells aiming to improve outcome of allogeneic hematopoietic stem cell transplantation. We have isolated MSC from umbilical cord (UC) exploring the interaction between CIK cells and UC-MSC. We found that UC-MSC could suppress CIK cells activity, when co-cultured in a cell-to-cell system. In addition, CIK cells could potentially lyse UC-MSC in a time and ratio dependent manner that could have implications for their in vivo use. Here we provide experimental data on the mutual interaction of CIK cells and UC-MSC, suggesting a negative interference when the two cell types are used in combination. In the light of our observations, when CIK and UC-MSC will be used in clinical trials, timing and sequencing of their infusion should be considered. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：159 / 165

页数：7

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