Anaerobic metabolism of L-phenylalanine via benzoyl-CoA in the denitrifying bacterium Thauera aromatica

被引:59
作者
Schneider, S
Mohamed, ME
Fuchs, G
机构
[1] UNIV FREIBURG, INST BIOL 2, D-79104 FREIBURG, GERMANY
[2] CAIRO UNIV, DEPT BOT, GIZA, EGYPT
关键词
Thauera aromatica; L-phenylalanine metabolism; phenylalanine transaminase; phenylpyruvate decarboxylase; phenylacetaldehyde;
D O I
10.1007/s002030050504
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The anaerobic metabolism of phenylalanine was studied in the denitrifying bacterium Thauera aromatica, a member of the beta-subclass of the Proteobacteria. Phenylalanine was completely oxidized and sen ed as the sole source of cell carbon. Evidence is presented that degradation proceeds via benzoyl-CoA as the central aromatic intermediate; the aromatic ring-reducing enzyme benzoyl-CoA reductase was present in cells grown on phenylalanine. Intermediates in phenylalanine oxidation to benzoyl-CoA were phenylpyruvate, phenylacetaldehyde, phenylacetate, phenylacetyl-CoA, and phenylglyoxylate. The required enzymes were detected in extracts of cells grown with phenylalanine and nitrate. Oxidation of phenylalanine to benzoyl-CoA was catalyzed by phenylalanine transaminase, phenylpyruvate decarboxylase, phenylacetaldehyde dehydrogenase (NAD(+)), phenylacetate-CoA ligase (AMP-forming), enzyme(s) oxidizing phenylacetyl-CoA to phenylglyoxylate with nitrate, and phenylglyoxylate:acceptor oxidoreductase. The capacity for phenylalanine oxidation to phenylacetate was induced during growth with phenylalanine. Evidence is provided that alpha-oxidation of phenylacetyl-CoA is catalyzed by a membrane-bound enzyme. This is the first report on the complete anaerobic degradation of an aromatic amino acid and the regulation of this process.
引用
收藏
页码:310 / 320
页数:11
相关论文
共 46 条
[11]   OXIDATION OF HOMOGENTISIC ACID BY CELL-FREE EXTRACTS OF A VIBRIO [J].
CHAPMAN, PJ ;
DAGLEY, S .
JOURNAL OF GENERAL MICROBIOLOGY, 1962, 28 (02) :251-&
[12]   CATABOLISM OF 3-HYDROXYPHENYLACETATE AND 4-HYDROXYPHENYLACETATE BY THE 3,4-DIHYDROXYPHENYLACETATE PATHWAY IN ESCHERICHIA-COLI [J].
COOPER, RA ;
SKINNER, MA .
JOURNAL OF BACTERIOLOGY, 1980, 143 (01) :302-306
[13]  
Curti B, 1992, CHEM BIOCH FLAVOENZY, V3, P69
[14]   OXIDATION OF PHENYLACETIC ACID BY A PSEUDOMONAS [J].
DAGLEY, S ;
WOOD, JM .
BIOCHIMICA ET BIOPHYSICA ACTA, 1965, 99 (02) :383-&
[15]   DIFFERENTIAL EXPRESSION OF ENZYME-ACTIVITIES INITIATING ANOXIC METABOLISM OF VARIOUS AROMATIC-COMPOUNDS VIA BENZOYL-COA [J].
DANGEL, W ;
BRACKMANN, R ;
LACK, A ;
MOHAMED, M ;
KOCH, J ;
OSWALD, B ;
SEYFRIED, B ;
TSCHECH, A ;
FUCHS, G .
ARCHIVES OF MICROBIOLOGY, 1991, 155 (03) :256-262
[16]   P-CRESOL FORMATION BY CELL-FREE-EXTRACTS OF CLOSTRIDIUM-DIFFICILE [J].
DARI, L ;
BARKER, HA .
ARCHIVES OF MICROBIOLOGY, 1985, 143 (03) :311-312
[17]   PURIFICATION, CHARACTERIZATION AND REGULATION OF A MONOMERIC L-PHENYLALANINE DEHYDROGENASE FROM THE FACULTATIVE METHYLOTROPH NOCARDIA SP-239 [J].
DEBOER, L ;
VANRIJSSEL, M ;
EUVERINK, GJ ;
DIJKHUIZEN, L .
ARCHIVES OF MICROBIOLOGY, 1989, 153 (01) :12-18
[18]   ANAEROBIC DEGRADATION OF TRANS-CINNAMATE AND OMEGA-PHENYLALKANE CARBOXYLIC-ACIDS BY THE PHOTOSYNTHETIC BACTERIUM RHODOPSEUDOMONAS-PALUSTRIS - EVIDENCE FOR A BETA-OXIDATION MECHANISM [J].
ELDER, DJE ;
MORGAN, P ;
KELLY, DJ .
ARCHIVES OF MICROBIOLOGY, 1992, 157 (02) :148-154
[19]  
ELDER DJE, 1992, FEMS MICROBIOL LETT, V98, P255, DOI 10.1111/j.1574-6968.1992.tb05524.x
[20]   END PRODUCTS OF METABOLISM OF AROMATIC AMINO-ACIDS BY CLOSTRIDIA [J].
ELSDEN, SR ;
HILTON, MG ;
WALLER, JM .
ARCHIVES OF MICROBIOLOGY, 1976, 107 (03) :283-288