Antibody response to the 45 kDa Candida albicans antigen in an animal model and potential role of the antigen in adherence

被引：17

作者：

Bujdakova, Helena ^{[1
]}

Paulovicova, Ema ^{[2
]}

Borecka-Melkusova, Silvia ^{[1
]}

Gasperik, Juraj ^{[3
]}

Kucharikova, Sona ^{[1
]}

Kolecka, Anna ^{[1
]}

Lell, Claudia ^{[4
]}

Jensen, Dorthe B. ^{[4
]}

Wuerzner, Reinhard ^{[4
]}

Chorvat, Dusan, Jr. ^{[5
]}

Pichova, Iva ^{[6
]}

机构：

[1] Comenius Univ, Fac Nat Sci, Dept Microbiol & Virol, Bratislava, Slovakia

[2] Slovak Acad Sci, Inst Chem, Bratislava, Slovakia

[3] Slovak Acad Sci, Inst Mol Biol, Bratislava 84251, Slovakia

[4] Innsbruck Med Univ, Dept Hyg Microbiol & Social Med, Innsbruck, Austria

[5] Ctr Int Laser, Dept Biophoton, Bratislava, Slovakia

[6] Acad Sci Czech Republic, Inst Organ Chem & Biochem, Prague, Czech Republic

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2008年 / 57卷 / 12期

关键词：

D O I：

10.1099/jmm.0.2008/001479-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Candida antigen CR3-RP (complement receptor 3-related protein) is supposed to be a 'mimicry' protein because of its ability to bind antibody directed against the alpha subunit of the mammalian CR3 (CD11b/CD18). This study aimed to (i) investigate the specific humoral isotypic response to immunization with CR3-RP in vivo in a rabbit animal model, and (ii) determine the role of CR3-RP in the adherence of Candida albicans in vitro using the model systems of buccal epithelial cells (BECs) and biofilm formation. The synthetic C. albicans peptide DINGGGATLPQ corresponding to 11 amino-acids of the CR3-RP sequence DINGGGATLPQALXQITGVIT, determined by N-terminal sequencing, was used for immunization of rabbits to obtain polyclonal anti-CR3-PR serum and for subsequent characterization of the humoral isotypic response of rabbits. A significant increase of IgG, IgA and IgM anti-CR3-RP specific antibodies was observed after the third (P<0.01) and the fourth (P<0.001) immunization doses. The elevation of IgA levels suggested peptide immunomodulation of the IgA1 subclass, presumably in coincidence with Candida epithelial adherence. Blocking CR3-RP with polyclonal anti-CR3-RP serum reduced the ability of Candida to adhere to BECs, in comparison with the control, by up to 35% (P<0.001), and reduced biofilm formation by 28% (P<0.001), including changes in biofilm thickness and integrity detected by confocal laser scanning microscopy. These properties of CR3-RP suggest that it has potential for future vaccine development.

引用

页码：1466 / 1472

页数：7

共 50 条

[31] Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR [J].

Nailis, Heleen ;

Coenye, Tom ;

Van Nieuwerburgh, Filip ;

Deforce, Dieter ;

Nelis, Hans J. .

BMC MOLECULAR BIOLOGY, 2006, 7

[32] Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors [J].

Netea, Mihai G. ;

Gow, Neil A. R. ;

Munro, Carol A. ;

Bates, Steven ;

Collins, Claire ;

Ferwerda, Gerben ;

Hobson, Richard P. ;

Bertram, Gwyneth ;

Hughes, H. Bleddyn ;

Jansen, Trees ;

Jacobs, Liesbeth ;

Buurman, Ed T. ;

Gijzen, Karlijn ;

Williams, David L. ;

Torensma, Ruurd ;

McKinnon, Alistair ;

MacCallum, Donna M. ;

Odds, Frank C. ;

Van der Meer, Jos W. M. ;

Brown, Alistair J. P. ;

Kullberg, Bart Jan .

JOURNAL OF CLINICAL INVESTIGATION, 2006, 116 (06) :1642-1650

[33] Critical role of Bcr1-dependent adhesins in C-albicans biofilm formation in vitro and in vivo [J].

Nobile, Clarissa J. ;

Andes, David R. ;

Nett, Jeniel E. ;

Smith, Frank J., Jr. ;

Yue, Fu ;

Phan, Quynh-Trang ;

Edwards, John E., Jr. ;

Filler, Scott G. ;

Mitchell, Aaron P. .

PLOS PATHOGENS, 2006, 2 (07) :636-649

[34]

Nosanchuk JD, 2003, J CLIN INVEST, V112, P1164, DOI [10.1172/JCI19361, 10.1172/JCI200319361]

[35] Antifungal and antitumor activities of a monoclonal antibody directed against a stress mannoprotein of Candida albicans [J].

Omaetxebarría, MJ ;

Moragues, MD ;

Elguezabal, N ;

Rodríguez-Alejandre, A ;

Brena, S ;

Schneider, J ;

Polonelli, L ;

Pontón, J .

CURRENT MOLECULAR MEDICINE, 2005, 5 (04) :393-401

[36] Immune response to Saccharomyces cerevisiae mannan conjugate in mice [J].

Paulovicová, E ;

Bystricky, S ;

Masárová, J ;

Machová, E ;

Mislovicová, D .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2005, 5 (12) :1693-1698

[37] Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells [J].

Phan, Quynh T. ;

Myers, Carter L. ;

Fu, Yue ;

Sheppard, Donald C. ;

Yeaman, Michael R. ;

Welch, William H. ;

Ibrahim, Ashraf S. ;

Edwards, John E., Jr. ;

Filler, Scott G. .

PLOS BIOLOGY, 2007, 5 (03) :543-557

[38] Candida albicans mannoprotein influences the biological function of dendritic cells [J].

Pietrella, D ;

Bistoni, G ;

Corbucci, C ;

Perito, S ;

Vecchiarelli, A .

CELLULAR MICROBIOLOGY, 2006, 8 (04) :602-612

[39] Immunoglobulins G could prevent adherence of Candida albicans to polystyrene and extracellular matrix components [J].

Rodier, MH ;

Imbert, C ;

Kauffmann-Lacroix, C ;

Daniault, G ;

Jacquemin, JL .

JOURNAL OF MEDICAL MICROBIOLOGY, 2003, 52 (05) :373-377

[40] Toll-like receptors and innate antifungal responses [J].

Roeder, A ;

Kirschning, CJ ;

Rupec, RA ;

Schaller, M ;

Korting, HC .

TRENDS IN MICROBIOLOGY, 2004, 12 (01) :44-49

← 1 2 3 4 5 →