Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans

被引:17
作者
Yao, Xinghao [1 ,2 ,4 ]
Wang, Tailin [1 ,2 ,5 ]
Wang, Huijuan [1 ,2 ,4 ]
Liu, Hongwei [1 ,2 ,4 ]
Liu, Shenghao [3 ]
Zhao, Qingang [1 ,2 ]
Chen, Kaoshan [1 ,2 ,4 ]
Zhang, Pengying [1 ,2 ,4 ]
机构
[1] Shandong Univ, Natl Glvcoengn Res Ctr, Jinan 250100, Shandong, Peoples R China
[2] Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China
[3] State Ocean Adm, Inst Oceanog 1, Marine Ecol Res Ctr, Qingdao 266061, Peoples R China
[4] Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Jinan 250100, Shandong, Peoples R China
[5] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
基金
中国国家自然科学基金;
关键词
abiotic stress; extreme environment; flavonoids; III POLYKETIDE SYNTHASES; ARABIDOPSIS-THALIANA; FLAVONOID BIOSYNTHESIS; GENETIC DIVERSITY; STRESS-TOLERANCE; LOCALIZATION; SALT; REGULATOR; MUTANTS; CLONING;
D O I
10.1017/S0954102018000470
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes (Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys(164), His(303) and Asn(336)) and two substrate recognition residues (Phe(215) and Phe(265)). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments.
引用
收藏
页码:23 / 33
页数:11
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