Continuous-flow in-droplet magnetic particle separation in a droplet-based microfluidic platform

被引：36

作者：

Lee, Hun ^{[1
]}

Xu, Linfeng ^{[1
]}

Ahn, Byungwook ^{[1
]}

Lee, Kangsun ^{[1
]}

Oh, Kwang W. ^{[1
]}

机构：

[1] SUNY Buffalo, SMALL, Dept Elect Engn, Buffalo, NY 14260 USA

来源：

MICROFLUIDICS AND NANOFLUIDICS | 2012年 / 13卷 / 04期

基金：

美国国家科学基金会;

关键词：

Magnetic particle manipulation; Magnetic field; Magnetic bead assay; Droplet merging; Droplet splitting; SYSTEMS; CHIP; TECHNOLOGY; VOLUME;

D O I：

10.1007/s10404-012-0978-7

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

This paper presents a continuous-flow in-droplet magnetic particle separation in a droplet-based microfluidic device for magnetic bead-based bioassays. Two functions, electrocoalescence and magnetic particle manipulation, are performed in this device. A pair of charging metallic needles is inserted into two aqueous channels of the device. By electrostatic force, two different solutions can be merged to be mixed at a junction of droplet generation. The manipulation of magnetic particles is achieved using an externally applied magnetic field. The magnetic particles are separated by the magnetic field to one side of the droplet and extracted by splitting the droplet into two daughter droplets: one contains the majority of the magnetic particles and the other is almost devoid of magnetic particles. The applicability of the continuous-flow in-droplet magnetic particle separation is demonstrated by performing a proof-of-concept immunoassay between streptavidin-coated magnetic beads and biotin labelled with fluorescence. This approach will be useful for various biological and chemical analyses and compartmentalization of small samples.

引用

页码：613 / 623

页数：11

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