Location of major histocompatibility complex class II molecules in rafts on dendritic cells enhances the efficiency of T-cell activation and proliferation

被引:34
作者
Eren, E
Yates, J
Cwynarski, K
Preston, S
Dong, R
Germain, C
Lechler, R
Huby, R
Ritter, M
Lombardi, G
机构
[1] Univ London Imperial Coll Sci & Technol, Dept Immunol, London, England
[2] AstraZeneca, Safety Assessment, Mol Toxicol Dept, Macclesfield, Cheshire, England
关键词
D O I
10.1111/j.1365-3083.2006.01700.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The existence of major histocompatibility complex (MHC) class II molecules in lipid rafts has been described in dendritic cells (DC); however, the importance of rafts in T-cell activation has not been clarified. In this study, the distribution of the lipid raft components (CD59 and GM1 ganglioside) in human monocyte-derived DC was investigated. DC had an even distribution of these components at the cell surface. In addition, raft-associated GM1 ganglioside colocalized with cross-linked MHC class II. This implies coaggregation of raft components with these MHC molecules, which may be important in the interaction between T cells and antigen-presenting cells. In studies carried out to investigate the effect of the DC : T-cell interaction on raft distribution, we found a clustering of the lipid raft component CD59 on DC at the synaptic interface, with associated activation of the interacting T cell. In an antigen-specific response between DC and CD4(+) T-cell clones, disruption of lipid rafts resulted in inhibition of both CD59 clustering and T-cell activation. This was most pronounced when limiting amounts of cognate peptide were used. Together, these data demonstrate the association of MHC class II with lipid rafts during DC : T-cell interaction and suggest an important role for DC lipid rafts in T-cell activation.
引用
收藏
页码:7 / 16
页数:10
相关论文
共 51 条
[31]  
2-1
[32]   Engagement of T cell receptor triggers its recruitment to low-density detergent-insoluble membrane domains [J].
Montixi, C ;
Langlet, C ;
Bernard, AM ;
Thimonier, J ;
Dubois, C ;
Wurbel, MA ;
Chauvin, JP ;
Pierres, M ;
He, HT .
EMBO JOURNAL, 1998, 17 (18) :5334-5348
[33]   Signalling via caveolin:: involvement in the cross-talk between phosphoinositolglycans and insulin [J].
Müller, G ;
Frick, W .
CELLULAR AND MOLECULAR LIFE SCIENCES, 1999, 56 (11-12) :945-970
[34]   Antibody cross-linking of the glycosylphosphatidylinositol-linked protein CD59 on hematopoietic cells induces signaling pathways resembling activation by complement [J].
Murray, EW ;
Robbins, SM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (39) :25279-25284
[35]   Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts [J].
Nguyen, DH ;
Hildreth, JEK .
JOURNAL OF VIROLOGY, 2000, 74 (07) :3264-3272
[36]   Rapid cycling of lipid raft markers between the cell surface and Golgi complex [J].
Nichols, BJ ;
Kenworthy, AK ;
Polishchuk, RS ;
Lodge, R ;
Roberts, TH ;
Hirschberg, K ;
Phair, RD ;
Lippincott-Schwartz, J .
JOURNAL OF CELL BIOLOGY, 2001, 153 (03) :529-541
[37]   Lipid rafts and B-cell activation [J].
Pierce, SK .
NATURE REVIEWS IMMUNOLOGY, 2002, 2 (02) :96-105
[38]   Glycolipid-enriched membrane domains are assembled into membrane patches by associating with the actin cytoskeleton [J].
Rodgers, W ;
Zavzavadjian, J .
EXPERIMENTAL CELL RESEARCH, 2001, 267 (02) :173-183
[39]   Composition of MHC class II-enriched lipid microdomains is modified during maturation of primary dendritic cells [J].
Setterblad, N ;
Roucard, C ;
Bocaccio, C ;
Abastado, JP ;
Charron, D ;
Mooney, N .
JOURNAL OF LEUKOCYTE BIOLOGY, 2003, 74 (01) :40-48
[40]   Signalling via MHC class II molecules modifies the composition of GEMs in APC [J].
Setterblad, N ;
Becart, S ;
Charron, D ;
Mooney, N .
SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2001, 54 (1-2) :87-92