Protein Diffusion in Polyelectrolyte Solutions

被引：0

作者：

Khandai, Santripti ^{[1
]}

Jena, Sidhartha S. ^{[1
]}

机构：

[1] Natl Inst Technol, Dept Phys, Rourkela 769008, Odisha, India

来源：

SOLID STATE PHYSICS, PTS 1 AND 2 | 2012年 / 1447卷

关键词：

Polyelectrolyte; Diffusion; FRAP; Non-dilute regime; PROBE DIFFUSION;

D O I：

10.1063/1.4709934

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

The diffusion of green fluorescent protein (GFP) in non-dilute polyelectrolyte solutions of Poly-L-lysine was studied using fluorescence recovery after photobleaching (FRAP) technique. The effect of background charges on probe diffusion of GFP was studied with varying ionic strength of the solution. With increase in polyelectrolyte concentration, increase in solution viscosity and decrease in probe diffusion coefficient was observed. At the same time, we observed the diffusion coefficient increased with increase in salt concentration, while solution viscosity decreased, indicating a competition between electrostatic force between background and probe and viscosity drag. When the probe diffusion coefficient was compared with the predicted Stokes-Einstein (S-E) relation, strong positive deviations were observed for all the solutions with highest deviation observed for solution with zero salt concentration.

引用

页码：169 / 170

页数：2

共 5 条

[1]

De Gennes P.-G, 1979, Scaling concepts in polymer physics

[2] Crowding effects on diffusion in solutions and cells [J].