Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum

被引:102
作者
Chen, Yun [1 ]
Gao, Qixun [1 ]
Huang, Mengmeng [1 ]
Liu, Ye [1 ]
Liu, Zunyong [1 ]
Liu, Xin [1 ]
Ma, Zhonghua [1 ]
机构
[1] Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
来源
SCIENTIFIC REPORTS | 2015年 / 5卷
基金
美国国家科学基金会;
关键词
MICRORNA-LIKE RNAS; ANTIVIRAL DEFENSE; NONCODING RNAS; KINASE GENE; GENOME; IDENTIFICATION; MACHINERY; PROTEIN; HETEROCHROMATIN; INTERFERENCE;
D O I
10.1038/srep12500
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The RNA interference (RNAi) plays a critical role in gene regulation in a variety of eukaryotic organisms. However, the role of RNAi remains largely unclear in plant pathogenic fungi. In this study, we explored the roles of core components of the RNAi pathway in Fusarium graminearum, the major causal agent of wheat head blight. Our results demonstrated that the hairpin RNA (hpRNA) can efficiently silence the expression level of target gene, and the argonaute protein FgAgo1 and dicer protein FgDicer2 are important in this silencing process. RNAi machinery was not involved in growth, abiotic stress and pathogenesis in F. graminearum under tested conditions. We firstly applied high-throughput sequencing technology to elucidate small RNA (17-40 nucleotides) (sRNA) transcriptome in F. graminearum, and found that a total of forty-nine micro-like-RNA (milRNA) candidates were identified in the wild-type and Delta FgDICER2, and twenty-four of them were FgDicer2-dependent. Fg-milRNA-4 negatively regulated expression of its target gene. Taken together, our results indicated that the hpRNA-induced gene silencing was a valuable genetic tool for exploring gene function in F. graminearum. FgAgo1 and FgDicer2 proteins played a critical role in the hpRNA mediated gene silencing process. In addition, FgDicer2 was involved in sRNA transcription and milRNA generation in this fungus.
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页数:13
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