Histone deacetylase 6 interference protects mice against experimental stroke-induced brain injury via activating Nrf2/HO-1 pathway

被引：15

作者：

Li, Jie ^{[1
]}

Zhao, Yanping ^{[1
]}

Shi, Junfeng ^{[1
]}

Ren, Zhanyun ^{[1
]}

Chen, Feng ^{[1
]}

Tang, Wuzhuang ^{[1
]}

机构：

[1] Yangzhou Univ, Affiliated Yixing Clin Sch, Sch Med, Dept Neurol,Affiliated Yixing Hosp,Jiangsu Univ, 75 Tongzhenguan Rd,Yicheng St, Yixing 214200, Jiangsu, Peoples R China

来源：

ANIMAL CELLS AND SYSTEMS | 2019年 / 23卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Histone deacetylase 6; Nrf2; HO-1; cerebral ischemia; reperfusion injury; oxidative stress; CEREBRAL-ARTERY OCCLUSION; CELLS;

D O I：

10.1080/19768354.2019.1601132

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cerebral stroke is a fatal disease with increasing incidence. The study was to investigate the role and mechanism of Histone deacetylase 6 (HDAC6) on experimental stroke-induced brain injury. The recombinant shRNA-HDAC6 or scramble shRNA lentivirus was transfected to ICR mice. Then, the ischemia/reperfusion injury (I/RI) mice were constructed using middle cerebral artery occlusion (MCAO) method. Brain TTC staining was used to determine infarct areas. Serum levels of oxidative stress-related factors were detected by enzyme linked immunosorbnent assay (ELISA). Realtime-qPCR (RT-qPCR) and Western blot were used to respectively detect mRNA and protein levels. HDAC6 was up-regulated in brain I/RI mice (MCAO group), and down-regulated again in MCAO mice transfected with shRNA-HDAC6 (MCAO + shRNA group). The infarct area of the MCAO mice was increased, neurological scores were higher, and serum protein levels of 3-NT, 4-HNE and 8-OHdG were higher. HDAC6 interference attenuated above effects. Though protein levels of Nrf2 and HO-1 in cytoplasm increased slightly in MCAO group, they increased significantly by HDAC6 interference. The protein levels of Nrf2 in cytoblast decreased significantly in MCAO group, and increased markedly by HDAC6 interference. HDAC6 interference protected mice against experimental stroke-induced brain injury via Nrf2/HO-1 pathway.

引用

页码：192 / 199

页数：8

共 30 条

[1] Role of Parkin and endurance training on mitochondrial turnover in skeletal muscle [J].

Chen, Chris Chin Wah ;

Erlich, Avigail T. ;

Hood, David A. .

SKELETAL MUSCLE, 2018, 8

[2] Extract of Rhus verniciflua stokes protects against renal ischemia-reperfusion injury by enhancing Nrf2-mediated induction of antioxidant enzymes [J].

Choi, Du Ri ;

Jeong, Ji Heun ;

Yu, Kwang-Sik ;

Lee, Nam-Seob ;

Jeong, Young-Gil ;

Kim, Do Kyung ;

Na, Chun Soo ;

Na, Dae Seung ;

Hwang, Won Min ;

Han, Seung-Yun .

EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (04) :3827-3835

[3] HDAC6 contributes to pathological responses of heart and skeletal muscle to chronic angiotensin-II signaling [J].

Demos-Davies, Kimberly M. ;

Ferguson, Bradley S. ;

Cavasin, Maria A. ;

Mahaffey, Jennifer H. ;

Williams, Sarah M. ;

Spiltoir, Jessica I. ;

Schuetze, Katherine B. ;

Horn, Todd R. ;

Chen, Bo ;

Ferrara, Claudia ;

Scellini, Beatrice ;

Piroddi, Nicoletta ;

Tesi, Chiara ;

Poggesi, Corrado ;

Jeong, Mark Y. ;

McKinsey, Timothy A. .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2014, 307 (02) :H252-H258

[4] Liraglutide Activates the Nrf2/HO-1 Antioxidant Pathway and Protects Brain Nerve Cells against Cerebral Ischemia in Diabetic Rats [J].

Deng, Caihong ;

Cao, Jun ;

Han, Jiangquan ;

Li, Jianguo ;

Li, Zhaohun ;

Shi, Ninghua ;

He, Jing .

COMPUTATIONAL INTELLIGENCE AND NEUROSCIENCE, 2018, 2018

[5]

Deng XL, 2000, ACTA PHARMACOL SIN, V21, P460

[6] Activation of Nrf2 and Hypoxic Adaptive Response Contribute to Neuroprotection Elicited by Phenylhydroxamic Acid Selective HDAC6 Inhibitors [J].

Gaisina, Irina N. ;

Lee, Sue H. ;

Kaidery, Navneet A. ;

Ben Aissa, Manel ;

Ahuja, Manuj ;

Smirnova, Natalya N. ;

Wakade, Sushama ;

Gaisin, Arsen ;

Bourassa, Megan W. ;

Ratan, Rajiv R. ;

Nikulin, Sergey V. ;

Poloznikov, Andrey A. ;

Thomas, Bobby ;

Thatcher, Gregory R. J. ;

Gazaryan, Irina G. .

ACS CHEMICAL NEUROSCIENCE, 2018, 9 (05) :894-900

[7] SUR1-TRPM4 channel activation and phasic secretion of MMP-9 induced by tPA in brain endothelial cells [J].

Gerzanich, Volodymyr ;

Kwon, Min Seong ;

Woo, Seung Kyoon ;

Ivanov, Alexander ;

Simard, J. Marc .

PLOS ONE, 2018, 13 (04)

[8] Stem cell-derived motor neurons from spinal and bulbar muscular atrophy patients [J].

Grunseich, Christopher ;

Zukosky, Kristen ;

Kats, Ilona R. ;

Ghosh, Laboni ;

Harmison, George G. ;

Bott, Laura C. ;

Rinaldi, Carlo ;

Chen, Ke-lian ;

Chen, Guibin ;

Boehm, Manfred ;

Fischbeck, Kenneth H. .

NEUROBIOLOGY OF DISEASE, 2014, 70 :12-20

[9] Susceptibility to Oxidative Stress Is Determined by Genetic Background in Neuronal Cell Cultures [J].

Gunther, Mattias ;

Al Nimer, Faiez ;

Piehl, Fredrik ;

Risling, Marten ;

Mathiesen, Tiit .

ENEURO, 2018, 5 (02)

[10] Ketogenic Diet Improves Brain Ischemic Tolerance and Inhibits NLRP3 Inflammasome Activation by Preventing Drp1-Mediated Mitochondrial Fission and Endoplasmic Reticulum Stress [J].

Guo, Min ;

Wang, Xun ;

Zhao, Yanxin ;

Yang, Qi ;

Ding, Hongyan ;

Dong, Qiang ;

Chen, Xingdong ;

Cui, Mei .

FRONTIERS IN MOLECULAR NEUROSCIENCE, 2018, 11

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