VEGF165 modulates proliferation, adhesion, migration and differentiation of cultured human outer root sheath cells from central hair follicle epithelium through VEGFR-2 activation in vitro

被引：24

作者：

Wu Xian-Jie ^{[1
]}

Zhu Jian-Wei ^{[1
]}

Jing Jing ^{[1
]}

Xue Dan ^{[2
]}

Liu Hai ^{[3
]}

Zheng Min ^{[1
]}

Lu Zhong-Fa ^{[1
]}

机构：

[1] Zhejiang Univ, Sch Med, Dept Dermatol, Affiliated Hosp 2, Hangzhou 310003, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Dept Plast Surg, Affiliated Hosp 2, Hangzhou 310003, Zhejiang, Peoples R China

[3] Zhejiang Univ, Sch Med, Dept Radiat Oncol, Sir Run Run Shaw Hosp, Hangzhou 310003, Zhejiang, Peoples R China

来源：

JOURNAL OF DERMATOLOGICAL SCIENCE | 2014年 / 73卷 / 02期

基金：

中国国家自然科学基金;

关键词：

VEGFR-2; Human outer root sheath cells; Central hair follicle epithelium; Proliferation; Differentiation; Migration; ENDOTHELIAL GROWTH-FACTOR; DERMAL PAPILLA CELLS; FACTOR RECEPTOR-1; FACTOR EXPRESSION; CANCER CELLS; STEM-CELLS; FLT-1; IDENTIFICATION; KERATINOCYTES; ANGIOGENESIS;

D O I：

10.1016/j.jdermsci.2013.10.002

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background: The functional state of vasculature is tightly controlled by vascular endothelial growth factor receptor-2 (VEGFR-2). Recent studies revealed that VEGFR-2 is expressed on hair follicle keratinocytes. Objective: We proposed to investigate its effect on proliferation, adhesion and migration of cultured human outer root sheath cells from central hair follicle epithelium. Methods: These studies were undertaken in vitro using human outer root sheath cells from central hair follicle epithelium, immunohistochemistry analysis, immunofluorescence microscopy, western blot analysis, MU, trans well analysis, and RT-PCR. Results: Our results show that VEGFR-2 is expressed in these cells in vivo and in vitro. Furthermore, proliferation and migration of cultured human outer root sheath cells from central hair follicle epithelium is increased by VEGF(165), while homotypic adhesion is decreased but heterotypic adhesion is increased. VEGF(165) upregulates integrin beta 1 but dowregulates Igr6 expression. In addition, phosphorylation of VEGFR-2, Erk1/2, c-Jun and p38, are increased following VEGF(165) treatment and these effects are reversed by a VEGFR-2 neutralizing antibody. Conclusion: Our results suggest a role of VEGF/VEGFR-2 beyond angiogenesis in hair follicle regulation. (C) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：152 / 160

页数：9