New innovation in order to recover the polyphenols of olive mill wastewater extracts for use as a biopesticide against the Euphyllura olivina and Aphis citricola

Results from physicochemical analysis of olive mill wastewater (OMW) have shown that total suspended solids (5.170 to 0.658 g/L), chemical oxygen demand (124.400 +/- A 25.442 g/L), biochemical oxygen demand (60.593 +/- A 6.276), total organic carbon (3.317 +/- A 0.350 g/L), and Kjeldahl nitrogen (0.126 +/- A 0.044 g/L) far exceed discharge standards. The concentrations of the major constituents of phenolic extracts of OMW can cause mortality of the pests Euphyllura olivina and Aphis citricola. Analysis of variance and use of the Sheffe test showed that exposure for 24 h to olive mill wastewater polyphenol extracts had a significant (P < 0.01) effect on survival of larval stages and adults, depending on concentration. LC50 and CL99 lethal concentrations were 3.642-18.864 g/L and 98.029-225.711 g/L respectively (confidence intervals). Recovery of polyphenols from vegetable waste for use as a biopesticide contributes to protection of citrus and olive trees against diseases, thus solving an environmental problem and avoiding use of synthetic insecticides.