Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction

被引：4
作者：
de Oliveira, Suelen Guedes [1 ]
Gonsalves Claudio, Erick Roberto [1 ]
de Almeida, Simone Alves [1 ]
Mengel, Vinicius [1 ]
da Silva, Fabricio Braganca [1 ]
Silva, Nyam Florencio [2 ]
Matted, Helder [1 ]
de Abreu, Glaucia Rodrigues [1 ]
机构：
[1] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Physiol Sci, Vitoria, ES, Brazil
[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Morphol, Vitoria, ES, Brazil
来源：
PLOS ONE | 2019年 / 14卷 / 04期
关键词：
HEART-FAILURE; ENDOTHELIAL DYSFUNCTION; OXIDATIVE STRESS; CORONARY; ANTIOXIDANTS; RESPONSES; OXIDASE; SYSTEM; IMPACT;
D O I：
10.1371/journal.pone.0215568
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to evaluate the effects of exercise training (ET) on the aortic vascular reactivity of ovariectomized and infarcted rats. The animals were divided into 5 groups: Control, Ovariectomized + SHAM sedentary (OVX+ SHAM(SED)), OVX+ SHAM and ET (OVX+ SHAM(ET)), OVX + Myocardial Infarction sedentary (OVX+ MISED), and OVX + MI and ET (OVX+ MIET). ET protocol (60 minutes/day, 5x/week) in a motorized treadmill began 15 days after MI and lasted 8 weeks. The endothelium-dependent and endothelium-independent vascular reactivity were evaluated as well as the role of the reactive oxygen species (ROS). Superoxide and nitric oxide (NO) production were analyzed in situ using DHE and DAF-2 fluorescence, respectively. The expression of gp91phox and of the antioxidant enzymes were evaluated by western blotting in the thoracic aorta samples. MI promoted a significant increase in the contractile response and impaired endothelium-mediated relaxation. However, ET prevented the impairment in the vascular reactivity in MI animals. In addition, the protein expression of gp91phox and superoxide production increased and the NO production decreased in the OVX+ MISED group but not in the OVX+ MIET group. Therefore, ET improves vascular reactivity in MI ovariectomized rats by preventing the increase in the expression of gp91phox and the decrease in the antioxidant enzymes, resulting in a normal ROS and NO production. Thus, ET can be an effective therapeutic strategy for improving the MI-induced vascular alterations in estrogen deficiency condition.
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页数：15
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