Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication

被引：6

作者：

Zhang, Pengfei ^{[1
,2
]}

Yu, Linyang ^{[1
,2
]}

Dong, Jianguo ^{[3
]}

Liu, Yanling ^{[1
,2
]}

Zhang, Leyi ^{[1
,2
]}

Liang, Pengshuai ^{[1
,2
]}

Wang, Lei ^{[1
,2
]}

Chen, Bin ^{[3
]}

Huang, Li ^{[3
]}

Song, Changxu ^{[1
,2
]}

机构：

[1] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Peoples R China

[2] South China Agr Univ, Natl Engn Ctr Swine Breeding Ind, Guangzhou 510642, Peoples R China

[3] Xinyang Agr & Forestry Univ, Sch Anim Husb & Med Engn, Xinyang 464000, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2020年 / 247卷

关键词：

PEDV; Coronavirus; Papain-like proteases; Poly(C) binding protein 2; Virus replication; Interaction; RESPIRATORY SYNDROME CORONAVIRUS; ANTIVIRAL SIGNALING PROTEIN; INTERFERON-PRODUCTION; HEPATITIS-VIRUS; IDENTIFICATION; RECOGNITION; DEGRADATION; INDUCTION; PATHWAY; 1-BETA;

D O I：

10.1016/j.vetmic.2020.108793

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine epidemic diarrhea virus (PEDV) belongs to the Alphacoronavirus genus in the Coronaviridae family. Similar to other coronaviruses, PEDV encodes two papain-like proteases. Papain-like protease (PLP)2 has been proposed to play a key role in antagonizing host innate immunity. However, the function of PLP1 remains unclear. In this study, we found that overexpression of PLP1 significantly promoted PEDV replication and inhibited production of interferon-beta. Immunoprecipitation and mass spectrometry were used to identify cellular interaction partners of PLP1. Host cell poly(C) binding protein 2 (PCBP2) was determined to bind and interact with PLP1. Both endogenous and overexpressed PCBP2 co-localized with PLP1 in the cytoplasm. Overexpression of PLP1 upregulated expression of PCBP2. Furthermore, overexpression of PCBP2 promoted PEDV replication. Silencing of endogenous PCBP2 using small interfering RNAs attenuated PEDV replication. Taken together, these data demonstrated that PLP1 negatively regulated the production of type 1 interferon by interacting with PCBP2 and promoted PEDV replication.

引用

页数：9

共 34 条

[1] The papain-like protease of severe acute respiratory syndrome coronavirus has deubiquitinating activity [J].

Barretto, N ;

Jukneliene, D ;

Ratia, K ;

Chen, ZB ;

Mesecar, AD ;

Baker, SC .

JOURNAL OF VIROLOGY, 2005, 79 (24) :15189-15198

[2] Cellular Poly(C) Binding Proteins 1 and 2 Interact with Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1β and Support Viral Replication [J].

Beura, Lalit K. ;

Dinh, Phat X. ;

Osorio, Fernando A. ;

Pattnaik, Asit K. .

JOURNAL OF VIROLOGY, 2011, 85 (24) :12939-12949

[3] Mitochondrial Antiviral Signaling Protein Plays a Major Role in Induction of the Fish Innate Immune Response against RNA and DNA Viruses [J].

Biacchesi, Stephane ;

LeBerre, Monique ;

Lamoureux, Annie ;

Louise, Yoann ;

Lauret, Emilie ;

Boudinot, Pierre ;

Bremont, Michel .

JOURNAL OF VIROLOGY, 2009, 83 (16) :7815-7827

[4] Proteolytic processing and deubiquitinating activity of papain-like proteases of human coronavirus NL63 [J].

Chen, Zhongbin ;

Wang, Yanhua ;

Ratia, Kiira ;

Mesecar, Andrew D. ;

Wilkinson, Keith D. ;

Baker, Susan C. .

JOURNAL OF VIROLOGY, 2007, 81 (11) :6007-6018

[5] Regulation of IRF-3-dependent innate immunity by the papain-like protease domain of the severe acute respiratory syndrome coronavirus [J].

Devaraj, Santhana G. ;

Wang, Nan ;

Chen, Zhongbin ;

Chen, Zihong ;

Tseng, Monica ;

Barretto, Naina ;

Lin, Rongtuan ;

Peters, Clarence J. ;

Tseng, Chien-Te K. ;

Baker, Susan C. ;

Li, Kui .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (44) :32208-32221

[6] Porcine Epidemic Diarrhea Virus Nucleocapsid Protein Antagonizes Beta Interferon Production by Sequestering the Interaction between IRF3 and TBK1 [J].

Ding, Zhen ;

Fang, Liurong ;

Jing, Huiyuan ;

Zeng, Songlin ;

Wang, Dang ;

Liu, Lianzeng ;

Zhang, Huan ;

Luo, Rui ;

Chen, Huanchun ;

Xiao, Shaobo .

JOURNAL OF VIROLOGY, 2014, 88 (16) :8936-8945

[7] Antagonistic Effects of Cellular Poly(C) Binding Proteins on Vesicular Stomatitis Virus Gene Expression [J].

Dinh, Phat X. ;

Beura, Lalit K. ;

Panda, Debasis ;

Das, Anshuman ;

Pattnaik, Asit K. .

JOURNAL OF VIROLOGY, 2011, 85 (18) :9459-9471

[8] Mechanisms of activation of interferon regulator factor 3: the role of C-terminal domain phosphorylation in IRF-3 dimerization and DNA binding [J].

Dragan, Anatoly I. ;

Hargreaves, Victoria V. ;

Makeyeva, Elena N. ;

Privalov, Peter L. .

NUCLEIC ACIDS RESEARCH, 2007, 35 (11) :3525-3534

[9] Identification of severe acute respiratory syndrome coronavirus replicase products and characterization of papain-like protease activity [J].

Harcourt, BH ;

Jukneliene, D ;

Kanjanahaluethai, A ;

Bechill, J ;

Severson, KM ;

Smith, CM ;

Rota, PA ;

Baker, SC .

JOURNAL OF VIROLOGY, 2004, 78 (24) :13600-13612

[10] Transmissible Gastroenteritis Virus Papain-Like Protease 1 Antagonizes Production of Interferon- β through Its Deubiquitinase Activity [J].

Hu, Xiaoliang ;

Tian, Jin ;

Kang, Hongtao ;

Guo, Dongchun ;

Liu, Jiasen ;

Liu, Dafei ;

Jiang, Qian ;

Li, Zhijie ;

Qu, Juanjuan ;

Qu, Liandong .

BIOMED RESEARCH INTERNATIONAL, 2017, 2017

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