The effect of Bcl-2 siRNA combined with miR-15a oligonucleotides on the growth of Raji cells

被引：5
作者：
Hu, Xiaomao [1 ]
Li, Wenyu [2 ]
Liu, Gexiu [1 ]
Wu, Hong [1 ]
Gao, Yangjun [1 ]
Chen, Shengting [3 ]
He, Dongmei [1 ]
Zhang, Yuan [1 ]
机构：
[1] Jinan Univ, Coll Med, Inst Hematol, Guangzhou 510632, Guangdong, Peoples R China
[2] Guangdong Gen Hosp, Guangdong Acad Med Sci, Ctr Canc, Guangzhou, Guangdong, Peoples R China
[3] Jinan Univ, Affiliated Hosp 1, Dept Hematol, Guangzhou 510632, Guangdong, Peoples R China
来源：
MEDICAL ONCOLOGY | 2013年 / 30卷 / 01期
基金：
中国国家自然科学基金;
关键词：
B-cell lymphoma 2 (Bcl-2); Small interfering RNA; Oligonucleotides; Raji cells; MiR-15a; Apoptosis; LEUKEMIC-CELLS; APOPTOSIS; EXPRESSION; MICRORNAS; ANTISENSE; GENES; PCR;
D O I：
10.1007/s12032-012-0430-6
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.
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页数：8
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