The effect of Bcl-2 siRNA combined with miR-15a oligonucleotides on the growth of Raji cells

被引：5

作者：

Hu, Xiaomao ^{[1
]}

Li, Wenyu ^{[2
]}

Liu, Gexiu ^{[1
]}

Wu, Hong ^{[1
]}

Gao, Yangjun ^{[1
]}

Chen, Shengting ^{[3
]}

He, Dongmei ^{[1
]}

Zhang, Yuan ^{[1
]}

机构：

[1] Jinan Univ, Coll Med, Inst Hematol, Guangzhou 510632, Guangdong, Peoples R China

[2] Guangdong Gen Hosp, Guangdong Acad Med Sci, Ctr Canc, Guangzhou, Guangdong, Peoples R China

[3] Jinan Univ, Affiliated Hosp 1, Dept Hematol, Guangzhou 510632, Guangdong, Peoples R China

来源：

MEDICAL ONCOLOGY | 2013年 / 30卷 / 01期

基金：

中国国家自然科学基金;

关键词：

B-cell lymphoma 2 (Bcl-2); Small interfering RNA; Oligonucleotides; Raji cells; MiR-15a; Apoptosis; LEUKEMIC-CELLS; APOPTOSIS; EXPRESSION; MICRORNAS; ANTISENSE; GENES; PCR;

D O I：

10.1007/s12032-012-0430-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.

引用

页数：8

共 27 条

[11] Small hairpin RNA targeting at Bcl-2 increases cytarabine-induced apoptosis in Raji cells [J].

He, Dongmei ;

Fang, Baoying .

ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2009, 41 (02) :131-136

[12] Dedication [J].

Kim, Kyoung-Woong .

ENVIRONMENTAL GEOCHEMISTRY AND HEALTH, 2012, 34 :1-1

[13] The role of Bcl-2 family members in tumorigenesis [J].

Kirkin, V ;

Joos, S ;

Zörnig, M .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2004, 1644 (2-3) :229-249

[14] Oblimersen bcl-2 antisense: Facilitating apoptosis in anticancer treatment [J].

Klasa, RJ ;

Gillum, AM ;

Klem, RE ;

Frankel, SR .

ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT, 2002, 12 (03) :193-213

[15] Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets [J].

Lewis, BP ;

Burge, CB ;

Bartel, DP .

CELL, 2005, 120 (01) :15-20

[16] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[17] Promises and challenges of targeting Bcl-2 anti-apoptotic proteins for cancer therapy [J].

O'Neill, J ;

Manion, M ;

Schwartz, P ;

Hockenbery, DM .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2004, 1705 (01) :43-51

[18] MicroRNAs in biological processes and carcinogenesis [J].

Osada, Hirotaka ;

Takahashi, Takashi .

CARCINOGENESIS, 2007, 28 (01) :2-12

[19] Analyzing real-time PCR data by the comparative CT method [J].

Schmittgen, Thomas D. ;

Livak, Kenneth J. .

NATURE PROTOCOLS, 2008, 3 (06) :1101-1108

[20] Widespread changes in protein synthesis induced by microRNAs [J].

Selbach, Matthias ;

Schwanhaeusser, Bjoern ;

Thierfelder, Nadine ;

Fang, Zhuo ;

Khanin, Raya ;

Rajewsky, Nikolaus .

NATURE, 2008, 455 (7209) :58-63

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