Structure of the neutral capsular polysaccharide of Acinetobacter baumannii NIPH146 that carries the KL37 capsule gene cluster

被引：33

作者：

Arbatsky, Nikolay P. ^{[1
]}

Shneider, Mikhail M. ^{[2
]}

Kenyon, Johanna J. ^{[5
,6
]}

Shashkov, Alexander S. ^{[1
]}

Popova, Anastasiya V. ^{[3
,4
]}

Miroshnikov, Konstantin A. ^{[2
]}

Volozhantsev, Nikolay V. ^{[4
]}

Knirel, Yuriy A. ^{[1
]}

机构：

[1] Russian Acad Sci, ND Zelinskii Organ Chem Inst, Moscow, Russia

[2] Russian Acad Sci, MM Shemyakin & YA Ovchinnikov Inst Bioorgan Chem, Moscow, Russia

[3] Moscow Inst Phys & Technol, Dolgoprudnyi, Moscow Region, Russia

[4] State Res Ctr Appl Microbiol & Biotechnol, Obolensk, Moscow Region, Russia

[5] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia

[6] Queensland Univ Technol, Sch Biomed Sci, Brisbane, Qld 4001, Australia

来源：

CARBOHYDRATE RESEARCH | 2015年 / 413卷

基金：

俄罗斯基础研究基金会; 英国医学研究理事会;

关键词：

Acinetobacter baumannii; Capsular polysaccharide structure; KL37 gene cluster; Glycosyltransferase; K locus; IDENTIFICATION; GLYCOSYLATION; RESISTANCE;

D O I：

10.1016/j.carres.2015.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Capsular polysaccharide (CPS) was isolated from Acinetobacter baumannii NIPH146, and the following structure of branched pentasaccharide repeating unit was established by sugar analyses along with 1D and 2D NMR spectroscopy: -> 4)-beta-D-GalpNAc-(1 -> 4)-alpha-D-Galp-(1 -> 6)-beta-D-Glcp-(1 -> 3)-beta-D-GalpNAc-(1 -> [GRAPHICS] beta-D-Glcp In comparison to most other known capsular polysaccharides of A. baumannii, the CPS studied is neutral and lacks any specific monosaccharide component. The synthesis, assembly and export of this structure could be attributed to genes in a novel capsule biosynthesis gene cluster, designated KL37, which was found in the NIPH146 genome. The CPS of A. baumannii NIPH146 shares the alpha-D-Galp-(1 -> 6)-beta-D-Glcp-(1 -> 3)-D-GalpNAc-(1 -> trisaccharide fragment with the CPS units of several A. baumannii strains, including ATCC 17978 and LUH 5537 that carry the KL3 and KL22 gene clusters, respectively. KL37 contains two genes for glycosyltransferases that are related to two glycosyltransferase genes present in both KL3 and KL22, and the encoded proteins could be tentatively assigned to linkages between sugars in the CPS repeat. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：12 / 15

页数：4

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