Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA's Profile and Cell Morphology in Human Colon Cancer Cell Lines

被引：8

作者：

Tutino, Valeria ^{[1
]}

Gigante, Isabella ^{[1
]}

Milella, Rosa Anna ^{[2
]}

De Nunzio, Valentina ^{[1
]}

Flamini, Riccardo ^{[3
]}

De Rosso, Mirko ^{[3
]}

Scavo, Maria Principia ^{[4
]}

Depalo, Nicoletta ^{[5
]}

Fanizza, Elisabetta ^{[5
,6
]}

Caruso, Maria Gabriella ^{[7
]}

Notarnicola, Maria ^{[1
]}

机构：

[1] Natl Inst Gastroenterol S de Bellis, Lab Nutr Biochem, Res Hosp, I-70013 Castellana Grotte, BA, Italy

[2] Council Agr Res & Econ, Res Ctr Viticulture & Enol, I-70010 Turi, BA, Italy

[3] Council Agr Res & Econ, Res Ctr Viticulture & Enol, I-31015 Conegliano, TV, Italy

[4] Natl Inst Gastroenterol S de Bellis, Personalized Med Lab, Res Hosp, I-70013 Castellana Grotte, BA, Italy

[5] Inst Chem Phys Proc IPCF CNR SS Bari, I-70125 Bari, BA, Italy

[6] Univ Bari Aldo Moro, Dipartimento Chim, I-70126 Bari, BA, Italy

[7] Natl Inst Gastroenterol S de Bellis, Ambulatory Clin Nutr, Res Hosp, I-70013 Castellana Grotte, BA, Italy

来源：

MOLECULES | 2020年 / 25卷 / 15期

关键词：

flavonoids; non-flavonoids; membrane PUFAs profile; cell morphology; human colon cancer cells; SEED EXTRACT; COLORECTAL-CANCER; 15-LIPOXYGENASE-1; POLYPHENOLS; APOPTOSIS; METASTASIS; SUPPRESSES; PREVENTION; CHEMISTRY; GROWTH;

D O I：

10.3390/molecules25153352

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-gamma), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-gamma were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-gamma gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways.

引用

页数：17

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