Accelerated High-Yield Generation of Limb-Innervating Motor Neurons from Human Stem Cells

被引:189
作者
Amoroso, Mackenzie W. [1 ,3 ,4 ,5 ]
Croft, Gist F. [1 ,3 ,4 ,5 ]
Williams, Damian J. [6 ]
O'Keeffe, Sean [7 ]
Carrasco, Monica A. [7 ]
Davis, Anne R. [8 ]
Roybon, Laurent [1 ,3 ,4 ,5 ]
Oakley, Derek H. [1 ,3 ,4 ,5 ]
Maniatis, Tom [7 ]
Henderson, Christopher E. [1 ,3 ,4 ,5 ]
Wichterle, Hynek [1 ,2 ,3 ,4 ,5 ]
机构
[1] Jenifer Estess Lab Stem Cell Res, Project ALS, New York, NY 10032 USA
[2] Columbia Univ, Coll Phys & Surg, Ctr Motor Neuron Biol & Dis, Dept Pathol, New York, NY 10032 USA
[3] Columbia Univ, Ctr Motor Neuron Biol & Dis, Dept Neurol, New York, NY 10032 USA
[4] Columbia Univ, Ctr Motor Neuron Biol & Dis, Dept Neurosci, New York, NY 10032 USA
[5] Columbia Univ, Columbia Stem Cell Initiat, New York, NY 10032 USA
[6] Columbia Univ, Dept Physiol & Cellular Biophys, New York, NY 10032 USA
[7] Columbia Univ, Dept Biochem & Biophys, New York, NY 10032 USA
[8] Columbia Univ, Dept Obstet & Gynecol, Med Ctr, New York, NY 10032 USA
基金
美国国家卫生研究院;
关键词
DIRECTED DIFFERENTIATION; HUMAN ES; HUMAN FIBROBLASTS; DIVERSITY; REQUIREMENT; ENRICHMENT; CONVERSION; PROTEINS; FOXP1; ALS;
D O I
10.1523/JNEUROSCI.0906-12.2013
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Human pluripotent stem cells are a promising source of differentiated cells for developmental studies, cell transplantation, disease modeling, and drug testing. However, their widespread use even for intensely studied cell types like spinal motor neurons is hindered by the long duration and low yields of existing protocols for in vitro differentiation and by the molecular heterogeneity of the populations generated. We report a combination of small molecules that within 3 weeks induce motor neurons at up to 50% abundance and with defined subtype identities of relevance to neurodegenerative disease. Despite their accelerated differentiation, motor neurons expressed combinations of HB9, ISL1, and column-specific markers that mirror those observed in vivo in human embryonic spinal cord. They also exhibited spontaneous and induced activity, and projected axons toward muscles when grafted into developing chick spinal cord. Strikingly, this novel protocol preferentially generates motor neurons expressing markers of limb-innervating lateral motor column motor neurons (FOXP1(+)/LHX3(-)). Access to high-yield cultures of human limb-innervating motor neuron subtypes will facilitate in-depth study of motor neuron subtype-specific properties, disease modeling, and development of large-scale cell-based screening assays.
引用
收藏
页码:574 / 586
页数:13
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