6′-O-Galloylpaeoniflorin Protects Human Keratinocytes Against Oxidative Stress-Induced Cell Damage

被引:17
作者
Yao, Cheng Wen
Piao, Mei Jing
Kim, Ki Cheon
Zheng, Jian
Cha, Ji Won
Hyun, Jin Won [1 ]
机构
[1] Jeju Natl Univ, Sch Med, Cheju 690756, South Korea
基金
新加坡国家研究基金会;
关键词
6 '-O-Galloylpaeoniflorin; HaCaT keratinocyte; Hydrogen peroxide; Oxidative stress; Reactive oxygen species; SINGLE-STRAND BREAKS; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; REDOX REGULATION; REACTIVE OXYGEN; DNA; ANTIOXIDANT; APOPTOSIS; CONSTITUENTS; BIOMARKERS;
D O I
10.4062/biomolther.2013.064
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
6'-O-galloylpaeoniflorin (GPF) is a galloylated derivate of paeoniflorin and a key chemical constituent of the peony root, a perennial flowering plant that is widely used as an herbal medicine in East Asia. This study is the first investigation of the cytoprotective effects of GPF against hydrogen peroxide (H2O2)-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated a significant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, H2O2-generated intracellular reactive oxygen species (ROS), the superoxide anion radical (O-2-), and the hydroxyl radical (center dot OH). GPF also safeguarded HaCaT keratinocytes against H2O2-provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector against ROS-mediated oxidative stress.
引用
收藏
页码:349 / 357
页数:9
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