Effects of amphiphilic star-shaped poly(ethylene glycol) polymers with a cholic acid core on human red blood cell aggregation

被引：15

作者：

Janvier, Florence ^{[1
,2
]}

Zhu, Julian X. X. ^{[2
,3
]}

Armstrong, Jonathan ^{[4
]}

Meiselman, Herbert J. ^{[4
]}

Cloutier, Guy ^{[1
,3
,5
]}

机构：

[1] Univ Montreal Hosp Res Ctr CRCHUM, Lab Biorheol & Med Ultrason, Montreal, PQ H2L 2W5, Canada

[2] Univ Montreal, Dept Chem, Montreal, PQ H3C 3J7, Canada

[3] Univ Montreal, Inst Biomed Engn, Montreal, PQ H3C 3J7, Canada

[4] Univ So Calif, Keck Sch Med, Dept Physiol & Biophys, Los Angeles, CA 90033 USA

[5] Univ Montreal, Dept Radiol Radiooncol & Nucl Med, Montreal, PQ H3C 3J7, Canada

来源：

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS | 2013年 / 18卷

基金：

加拿大健康研究院; 美国国家卫生研究院;

关键词：

Star-shaped poly(ethylene glycol); Erythrocytes; Aggregation; Cholic acid; Blood rheology; Blood viscosity; BILE-ACIDS; COMPLEMENT ACTIVATION; DIABETES-MELLITUS; VISCOSITY; POLOXAMER-188; DERIVATIVES; LIGHT; INHIBITION; SCATTERING; RHEOLOGY;

D O I：

10.1016/j.jmbbm.2012.11.008

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Elevated red blood cell (RBC) aggregation increases low-shear blood viscosity and is closely related to several pathophysiological diseases such as atherosclerosis, thrombosis, diabetes, hypertension, cancer, and hereditary chronic hemolytic conditions. Non-ionic linear polymers such as poly(ethylene glycol) (PEG) and Pluronic F68 have shown inhibitory effects against EEC aggregation. However, hypersensitivity reactions in some individuals, attributed to a diblock component of Pluronic F68, have been reported. Therefore, we investigated the use of an amphiphilic star-shaped PEG polymer based on a cholic acid core as a substitute for Pluronics to reduce RBC aggregation. Cholic acid is a natural bile acid produced in the human liver and therefore should assure biocompatibility. Cholic acid based PEG polymers, termed CA(PEG)(4), were synthesized by anionic polymerization. Size exclusion chromatography indicated narrow mass distributions and hydrodynamic radii less than 2 nm were calculated. The effects of CA(PEG)(4) on human EEC aggregation and blood viscosity were investigated and compared to linear PEGs by light transmission aggregometry. Results showed optimal reduction of EEC aggregation for molar masses between 10 and 16 kDa of star-shaped CA(PEG)(4) polymers. Cholic acid based PEG polymers affect the rheology of erythrocytes and may find applications as alternatives to linear PEG or Pluronics to improve blood fluidity. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：100 / 107

页数：8

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