Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni

被引：63

作者：

Dillon, GP

Feltwell, T

Skelton, JP

Ashton, PD

Coulson, PS

Quail, MA

Nikolaidou-Katsaridou, N

Wilson, RA

Ivens, AC ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Pathogen Microarrays Grp, Hinxton CB10 1SA, England

[2] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2006年 / 36卷 / 01期

基金：

英国惠康基金;

关键词：

blood fluke; life cycle; transcriptome; secretion; membrane proteins; Schistosoma;

D O I：

10.1016/j.ijpara.2005.10.008

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：1 / 8

页数：8

共 18 条

[1] X-ray structure of Na-ASP-2, a pathogenesis-related-1 protein from the nematode parasite, Necator americanus, and a vaccine antigen for human hookworm infection [J].