Cellular Uptake Behavior of Doxorubicin-Conjugated Nanodiamond Clusters for Efficient Cancer Therapy

被引：24

作者：

Ryu, Tae-Kyung ^{[1
]}

Lee, Gyoung-Ja ^{[2
]}

Rhee, Chang-Kyu ^{[2
]}

Choi, Sung-Wook ^{[1
]}

机构：

[1] Catholic Univ Korea, Dept Biotechnol, Bucheon Si 420743, Gyeonggi Do, South Korea

[2] Korea Atom Energy Res Inst, Nucl Mat Res Div, Taejon 305353, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2015年 / 15卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

cancer therapy; cellular uptake; doxorubicin; fluidic device; nanodiamond cluster; WALL CARBON NANOTUBES; GRAPHENE OXIDE; DRUG-DELIVERY; INTRATRACHEAL INSTILLATION; PHOTOTHERMAL THERAPY; PULMONARY TOXICITY; NANOPARTICLES; RELEASE; BIODISTRIBUTION; THERAPEUTICS;

D O I：

10.1002/mabi.201500176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes the design and fabrication of doxorubicin (Dox)-conjugated nanodiamond (ND) clusters with controlled sizes and cellular uptake behaviors of free Dox and Dox-conjugated ND clusters. The ND clusters with an average size of 45.84nm exhibited a higher amount of cellular uptake as compared to the ND clusters with larger sizes. The amount of Dox taken up as free Dox increased initially and then decreased over time. In contrast, the amount of Dox taken up as Dox-ND clusters continuously increased and reached a plateau, resulting in high ablation efficiency. At the same Dox concentration, the cell viabilities after treatment with free Dox and Dox-ND clusters were 26.38 and 5.31%, respectively. The Dox-ND clusters potentially could be employed as efficient drug carriers for efficient cancer therapy.

引用

页码：1469 / 1475

页数：7

共 38 条

[1] Stabilized Magnetic Cerasomes for Drug Delivery [J].

Cao, Zhong ;

Yue, Xiuli ;

Li, Xiaoda ;

Dai, Zhifei .

LANGMUIR, 2013, 29 (48) :14976-14983

[2] Nanodiamond-Mediated Delivery of Water-Insoluble Therapeutics [J].

Chen, Mark ;

Pierstorff, Erik D. ;

Lam, Robert ;

Li, Shu-You ;

Huang, Houjin ;

Osawa, Eiji ;

Ho, Dean .

ACS NANO, 2009, 3 (07) :2016-2022

[3] Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes [J].

Chithrani, B. Devika ;

Chan, Warren C. W. .

NANO LETTERS, 2007, 7 (06) :1542-1550

[4] Nanodiamond Therapeutic Delivery Agents Mediate Enhanced Chemoresistant Tumor Treatment [J].

Chow, Edward K. ;

Zhang, Xue-Qing ;

Chen, Mark ;

Lam, Robert ;

Robinson, Erik ;

Huang, Houjin ;

Schaffer, Daniel ;

Osawa, Eiji ;

Goga, Andrei ;

Ho, Dean .

SCIENCE TRANSLATIONAL MEDICINE, 2011, 3 (73)

[5] Adhesion and wrapping in colloid-vesicle complexes [J].

Deserno, M ;

Gelbart, WM .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (21) :5543-5552

[6] Mechanics of receptor-mediated endocytosis [J].

Gao, HJ ;

Shi, WD ;

Freund, LB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9469-9474

[7] Biodistribution of fluoresceinated dextran using novel nanoparticles evading reticuloendothelial system [J].

Gaur, U ;

Sahoo, SK ;

De, TK ;

Ghosh, PC ;

Maitra, A ;

Ghosh, PK .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 202 (1-2) :1-10

[8] Selective Photothermal Therapy for Mixed Cancer Cells Using Aptamer-Conjugated Nanorods [J].

Huang, Yu-Fen ;

Sefah, Kwame ;

Bamrungsap, Suwussa ;

Chang, Huan-Tsung ;

Tan, Weihong .

LANGMUIR, 2008, 24 (20) :11860-11865

[9] Uniform polydimethylsiloxane beads coated with polydopamine and their potential biomedical applications [J].

Jun, Dae-Ryong ;

Moon, Seung-Kwan ;

Choi, Sung-Wook .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 121 :395-399

[10] Plasmonic Nanopowders for Photothermal Therapy of Tumors [J].

Khlebtsov, Boris N. ;

Panfilova, Elizaveta V. ;

Terentyuk, Georgy S. ;

Maksimova, Irina L. ;

Ivanov, Andrei V. ;

Khlebtsov, Nikolai G. .

LANGMUIR, 2012, 28 (24) :8994-9002

← 1 2 3 4 →